Сверхсшитые полимерные сорбенты, модифицированные 4-винилпиридином: синтез, адсорбционные свойства и применение для выделения полимерной соли цефуроксима в режиме флэш-хроматографии

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Abstract

Синтезированы сверхсшитые полимерные сорбенты на основе терполимеров стирола, дивинилбензола и 4-винилпиридина. Пористая и химическая структура сорбентов исследована с помощью методов низкотемпературной адсорбции–десорбции азота и инфракрасной спектроскопии. Определены адсорбционные характеристики сорбентов по отношению к цефуроксиму в статических и динамических условиях. Установлено, что введение 4-винилпиридина в полимерную матрицу сорбентов способствует усиленной адсорбции цефуроксима. Показана возможность применения пиридинсодержащих сверхсшитых полимерных сорбентов для разделения соли поли(акриламид-со-2-аминоэтилметакрилат)а с цефуроксимом и свободного цефуроксима в режиме флэш-хроматографии. Чистота полимерной соли цефуроксима после выделения подтверждена с помощью высокоэффективной жидкостной хроматографии в эксклюзионном режиме.

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About the authors

Антон Сергеевич Кириллов

Институт высокомолекулярных соединений РАН

Author for correspondence.
Email: a.kirillov2622@gmail.com
ORCID iD: 0000-0003-1350-6775

м.н.с.

Russian Federation, 199004, г. Санкт-Петербург, Большой пр. В. О., д. 31

Артем Андреевич Вагин

Институт высокомолекулярных соединений РАН

Email: a.kirillov2622@gmail.com
ORCID iD: 0000-0002-3891-3601

м.н.с.

Russian Federation, 199004, г. Санкт-Петербург, Большой пр. В. О., д. 31

Максим Сергеевич Борисенко

Институт высокомолекулярных соединений РАН

Email: a.kirillov2622@gmail.com
ORCID iD: 0000-0001-9335-5932

к.х.н., н.с.

Russian Federation, 199004, г. Санкт-Петербург, Большой пр. В. О., д. 31

Наталья Николаевна Шевченко

Институт высокомолекулярных соединений РАН

Email: a.kirillov2622@gmail.com
ORCID iD: 0000-0003-2471-297X

к.х.н., с.н.с.

Russian Federation, 199004, г. Санкт-Петербург, Большой пр. В. О., д. 31

Валерий Дмитриевич Красиков

Институт высокомолекулярных соединений РАН

Email: a.kirillov2622@gmail.com
ORCID iD: 0000-0002-2137-7144

д.х.н., г.н.с.

Russian Federation, 199004, г. Санкт-Петербург, Большой пр. В. О., д. 31

Николай Иванович Горшков

Институт высокомолекулярных соединений РАН

Email: a.kirillov2622@gmail.com
ORCID iD: 0000-0003-1104-8264

к.х.н., с.н.с.

Russian Federation, 199004, г. Санкт-Петербург, Большой пр. В. О., д. 31

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Supplementary files

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2. Fig. 1. IR spectra of a hypercrosslinked copolymer of styrene and divinylbenzene (1) and hypercrosslinked terpolymers containing 5 (2), 12 (3) and 20 mol% (4) 4-vinylpyridine.

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3. Fig. 2. Isotherms of equilibrium adsorption of cefuroxime from an aqueous solution on a hypercrosslinked copolymer of styrene and divinylbenzene (1) and hypercrosslinked terpolymers containing 20 (2), 5 (3) and 12 mol% (4) of 4-vinylpyridine; c0 = 100–500 mg L–1, V = 8 ml, m = 8 mg.

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4. Fig. 3. Output retention curves of cefuroxime from an aqueous solution on a hyper-cross-linked copolymer (1) and hyper-cross-linked terpolymers containing 20 (2), 12 (3) and 5 mol% (4) 4-vinylpyridine; W = 0.5 ml min–1, m = 200 mg, c0 = 300 mg L–1.

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5. Fig. 4. Chromatograms of cefuroxime (1), poly(acrylamide-co-2-aminoethyl methacrylate)a (2), polymer salt of cefuroxime based on poly(acrylamide-co-2-aminoethyl methacrylate)a before (3) and after (4) separation into hyper-crosslinked polymer sorbent containing 5 mol% 4-vinylipyridine. Absorbance at wavelengths 210 (black line) and 280 nm (red line). Column PSS Suprema, 10 µm, 8 × 300 mm; eluent 0.2 M aqueous solution of NaCl; flow rate 1 ml min–1.

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6. Scheme 1

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7. Scheme 2

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