Expression of muscle-specific genes in atlantic salmon fingerlings (SALMO SALAR L.) in the conditions of aquaculture under the influence of different lighting and feeding regimes

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The expression level of myosin heavy and light chains genes (myhc, mlc-2), transcription myogenic regulation factors (myf5, myog, myod1 paralogs), myostatin paralogs and insulin-like growth factors (igf1 and igf2) in the muscles of salmon fingerlings artificially grown under different lighting and feeding conditions at a constant water temperature was studied in the region of North Ossetia-Alania. The combined effect of lighting and feeding modes was reflected in the expression of the genes myhc, mlc-2, myod1a, myf5, myod1b, myod1c. Thus, individuals reared under constant lighting conditions were distinguished by higher levels of expression of the myf5, myhc and mlc-2 genes compared to fish in natural light, which also corresponded to their higher growth rates. The expression of IGF genes varied in different ways throughout the experiment: the expression level of the igf1 gene increased, and the igf2 gene first increased after a month of study, then decreased on the second month. The results indicate differences in the processes of myogenesis regulation depending on lighting and feeding conditions.

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M. Kuznetsova

Institute of Biology of the Karelian Research Centre of the Russian Academy of Sciences

编辑信件的主要联系方式.
Email: kuznetsovamvi@yandex.ru
俄罗斯联邦, Petrozavodsk

M. Rodin

Institute of Biology of the Karelian Research Centre of the Russian Academy of Sciences

Email: kuznetsovamvi@yandex.ru
俄罗斯联邦, Petrozavodsk

N. Shulgina

Institute of Biology of the Karelian Research Centre of the Russian Academy of Sciences

Email: kuznetsovamvi@yandex.ru
俄罗斯联邦, Petrozavodsk

M. Krupnova

Institute of Biology of the Karelian Research Centre of the Russian Academy of Sciences

Email: kuznetsovamvi@yandex.ru
俄罗斯联邦, Petrozavodsk

A. Kuritsyn

Institute of Biology of the Karelian Research Centre of the Russian Academy of Sciences

Email: kuznetsovamvi@yandex.ru
俄罗斯联邦, Petrozavodsk

S. Murzina

Institute of Biology of the Karelian Research Centre of the Russian Academy of Sciences

Email: kuznetsovamvi@yandex.ru
俄罗斯联邦, Petrozavodsk

N. Nemova

Institute of Biology of the Karelian Research Centre of the Russian Academy of Sciences

Email: kuznetsovamvi@yandex.ru
俄罗斯联邦, Petrozavodsk

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2. Fig. 1. The relative level of gene expression (in units) of myhc (a), mlc‑2 (b), myf5 (c), myog (d) in the white muscles of Atlantic salmon raised in groups with different lighting and feeding modes during the study period: September – values before the experiment; group 1 – ”24C KK“ – constant lighting mode , round–the–clock feeding; group 2 - ”EstF CD“ natural photoperiod, feeding during daylight hours; group 3 - "24C CD” – constant lighting mode, feeding in the daytime. The differences are significant at p < 0.05 (criterion Kraskel–Wallis, Mann–Whitney criterion, adjusted for multiple comparisons): * – compared with group 1, # – compared with group 2, a – compared with values in September, b – compared with values in October in the corresponding group.

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3. Fig. 2. The relative level of gene expression (units) of myod1a (a), myod1b (b), myod1c (c) in the white muscles of Atlantic salmon grown in groups with different lighting and feeding modes during the study period: September – values before the experiment; group 1 – "24C KK” – constant lighting mode, round-the-clock feeding; group 2 – “EstF CD” natural photoperiod, feeding in the daytime; group 3 – “24C CD” – constant illumination mode, feeding in the daytime. The differences are significant at p < 0.05 (Kraskel–Wallis criterion, Mann–Whitney criterion, adjusted for multiple comparisons): * – compared with group 1, # – compared with group 2, a - compared with values in September, b – compared with values in October in the corresponding the group.

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4. Fig. 3. The relative level of gene expression (units) mstn1a (a), mstn1b (b) in the white muscles of Atlantic salmon raised in groups with different lighting and feeding modes during the study period: September - values before the experiment; group 1 – “24C KK” – lighting mode constant, round-the-clock feeding; group 2 – “EstF CD” natural photoperiod, feeding in the daytime; group 3 – “24C CD” – constant lighting mode , feeding in the daytime. The differences are significant at p < 0.05 (Kraskel–Wallis criterion, criterion Mann–Whitney adjusted for multiple comparisons): * – compared with group 1, a – compared with values in September, b – compared with values in October in the corresponding group.

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5. Fig. 4. The relative level of gene expression (units) igf1 (a), igf2 (b) in the white muscles of Atlantic salmon grown in groups with different lighting and feeding modes during the study period: September - values before the experiment; group 1 – “24C KK” – lighting mode constant, round-the-clock feeding; group 2 – “EstF CD” natural photoperiod, feeding in the daytime; group 3 – “24C CD” – constant lighting mode , feeding in the daytime. The differences are significant at p < 0.05 (Kraskel–Wallis criterion, criterion Mann–Whitney adjusted for multiple comparisons): * – compared with group 1, a – compared with values in September, b – compared with values in October in the corresponding group.

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