GLOMERULAR IBA-1-HUMAN KIDNEY IMMUNOPOSITIVE CELLS

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Abstract

Inflammatory processes occurring in the kidney, as a rule, lead to the development of chronic renal failure. Macrophages play a key role in this. They determine the course of the inflammatory process and the formation of fibrous tissue in trauma and glomerulonephritis, as well as the intensity of regeneration and repair of the kidney in case of damage. The most commonly used marker for macrophage imaging is the Iba-1 protein, and therefore the aim of the work was to identify Iba-1 immunopositive cells in the human kidney and determine their predominant localization. The study used kidney samples from men and women of different ages without renal pathology and kidneys from sexually mature male Wistar rats. An immunohistochemical study showed the presence of Iba-1 immunopositive macrophages in the interstitial connective tissue of the kidney of all analyzed samples. Along with this, the Iba-1 protein was present in human glomerular podocytes and was absent in similar structures in rats. The data obtained indicate the specific features of the expression of this protein in the kidney, which should be taken into account when modeling pathology and interpreting the results of biochemical studies.

About the authors

O. V. Kirik

Institute of Experimental Medicine

St. Petersburg, Russia

O. S. Alekseeva

Institute of Experimental Medicine; Sechenov Institute of Evolutionary Physiology and Biochemistry of the Russian Academy of Sciences

Email: osa72@inbox.ru
St. Petersburg, Russia

V. A. Razenkova

Institute of Experimental Medicine

St. Petersburg, Russia

I. A. Nikitina

Institute of Experimental Medicine

St. Petersburg, Russia

Yu. M. Podsumkova

Institute of Experimental Medicine

St. Petersburg, Russia

A. A. Beketova

Institute of Experimental Medicine

St. Petersburg, Russia

D. E. Korzhevsky

Institute of Experimental Medicine

St. Petersburg, Russia

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