SHORT-TERM EFFECTS OF LEAD EXPOSURE ON CARDIOVASCULAR CELLS OF THE FROG Rana ridibunda in situ

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Abstract

Lead (Pb2+) is a common and environmentally hazardous element. Lead poses a significant health risk because it can mimic the effects of metal ions such as zinc and calcium, which are involved in cellular signaling and metabolism. Available data re the effects of Pb2+ on myocardial and vascular function are contradictory. Moreover, the effect of Pb2+ on store-operated calcium entry (SOCE) in cardiovascular cells has not been studied. Therefore, the aim of this study was to investigate the short-term effects of Pb2+ on spontaneous contractility of cardiac muscle and blood vessels in situ and determine its toxic effects on SOCE. We used isolated atrial and aortic muscle preparations of male frogs, Rana ridibunda. Pb2+ at concentrations of 0.1 and 0.4 mM had a significant negative inotropic effect, and increased stiffness, and reduced the amplitude of KCl-induced aortic contraction. Pb2+ reversibly blocked SOCE in aortic smooth muscle cells, which may exacerbate its toxic effects on organisms.

About the authors

K. V Sobol

Sechenov Institute of Evolutionary Physiology and Biochemistry of the Russian Academy of Sciences

Email: peep9@yandex.ru
St. Petersburg, Russia

I. V Shemarova

Sechenov Institute of Evolutionary Physiology and Biochemistry of the Russian Academy of Sciences

St. Petersburg, Russia

V. P Nesterov

Sechenov Institute of Evolutionary Physiology and Biochemistry of the Russian Academy of Sciences

St. Petersburg, Russia

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