Embryo-conditioned media affect the functional state of endothelial cells

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Abstract

Currently, the main method of selecting high-quality embryos in assisted reproduction is based on morphological evaluation. In order to standardize the selection process and increase transfer efficiency, it is necessary to develop other objective, non-invasive methods. One potential approach is the assessment of soluble factors in media samples collected during embryo incubation. The aim of the study was to determine the levels of IL-1β, IL-4, IL-5, IL-6, IL-8, IL-10, IP-10, G-CSF, and GM-CSF in embryo-conditioned media, as well as assess the effect of these media on the functional activity of endothelial cells. We did not find stable levels of these cytokines in the embryo-conditioned medium after 2 days of incubation. Nevertheless, the media obtained from embryos of quality A and AB, consisting of 3 or 4 cells, had an effect on the proliferation and migration of endothelial cells. In the future, it will be necessary to identify the nature of active molecules present in embryo-conditioned media in order to determine their potential as markers for selecting the best embryos.

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E. V. Tyshchuk

Research Institute of Obstetrics, Gynecology, and Reproductology named after D.O. Ott

Author for correspondence.
Email: lisatyshchuk@yandex.ru
Russian Federation, Saint Petersburg

M. S. Zementova

Research Institute of Obstetrics, Gynecology, and Reproductology named after D.O. Ott

Email: lisatyshchuk@yandex.ru
Russian Federation, Saint Petersburg

E. M. Komarova

Research Institute of Obstetrics, Gynecology, and Reproductology named after D.O. Ott

Email: lisatyshchuk@yandex.ru
Russian Federation, Saint Petersburg

E. A. Lesik

Research Institute of Obstetrics, Gynecology, and Reproductology named after D.O. Ott

Email: lisatyshchuk@yandex.ru
Russian Federation, Saint Petersburg

K. V. Ob’edkova

Research Institute of Obstetrics, Gynecology, and Reproductology named after D.O. Ott

Email: lisatyshchuk@yandex.ru
Russian Federation, Saint Petersburg

O. N. Bespalova

Research Institute of Obstetrics, Gynecology, and Reproductology named after D.O. Ott

Email: lisatyshchuk@yandex.ru
Russian Federation, Saint Petersburg

S. A. Selkov

Research Institute of Obstetrics, Gynecology, and Reproductology named after D.O. Ott

Email: lisatyshchuk@yandex.ru
Russian Federation, Saint Petersburg

D. I. Sokolov

Research Institute of Obstetrics, Gynecology, and Reproductology named after D.O. Ott

Email: lisatyshchuk@yandex.ru
Russian Federation, Saint Petersburg

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2. Fig. 1. Cell proliferation (a), the number of migrated cells (b) and the residual area of ​​the desquamated cell layer (c). 1:1 DMEM/F12 + G-TL 2.5% FBS — the level of cell migration in the presence of DMEM/F12 medium and G-TL medium with 2.5% FBS; DMEM/F12 0% FBS — the level of migration in the presence of a medium with 0% FBS; DMEM/F12 2.5% FBS — the level of migration in the presence of a medium with 2.5% FBS; DMEM/F12 10% FBS — the level of migration in the presence of a medium with 10% FBS. Significance of differences: * — p <0.05; *** — p <0.001.

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3. Fig. 2. Cell proliferation in the presence of FCS. (a) — FCS of embryos of different quality (A, AB, B or C) and medium with 2.5% FCS; (b) — FCS of embryos of different cellularity (2, 4, 5 or 6 blastomeres); (c) — FCS of embryos of different quality and cellularity 2 or 4 blastomeres; (d) — FCS of quality A embryos of different cellularity (2, 4 or 6 blastomeres); Control — cell proliferation level in DMEM/F12 and G-TL media in a 1:1 ratio and 2.5% FCS. Significance of differences: ** — p < 0.005.

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4. Fig. 3. The number of migrated cells and the residual area of ​​the desquamated cell layer in the presence of FES. (a) — FES of embryos of different quality (A, AB, B, or C); (b) — FES of quality B embryos of different cellularity (4, 5, or 8 blastomeres); (c) — FES of quality C embryos of different cellularity (2, 3, or 4 blastomeres); (d) — FES of quality A embryos of different cellularity (2, 3, 4, 5, or 6 blastomeres); (e) — FES of embryos of different cellularity (2, 3, 4, 5, 6, or 8 blastomeres); (f) — FES of embryos of different quality and cellularity. Control — the level of cell proliferation in DMEM/F12 and G-TL media in a ratio of 1:1 and 2.5% FES. Significance of differences: * — p <0.05; ** — p < 0.01; *** — p <0.001.

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