METABOLIC ADAPTATION OF FISH INTESTINAL HELMINTHS: INHIBITORY ABILITY TOWARDS PROTEASES IN CESTODES TRIAENOPHORUS NODULOSUS

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Resumo

The inhibitory ability towards proteases was studied in the extracts of plerocercoids from the liver of perch (Perca fluviatilis Linnaeus), as well as in the excretory-secretory products and extracts of mature and immature cestodes Triaenophorus nodulosus (Pallas, 1781) from the intestine of pike (Esox lucius Linnaeus). It was found that excretory-secretory products and extracts of both mature and immature T. nodulosus display varying degrees of inhibitory ability against proteases. A significant decrease in activity was noted under the action of the studied samples on the activity of commercial trypsin. Tapeworm extracts exhibit a greater inhibitory ability compared to excretory-secretory products. At the same time, the extract of immature worms inhibits the activity of proteases more strongly than that of mature ones. The inhibitory ability is more closely associated with the extract of worms lacking the brush border than with the tegumental brush border fraction.

Sobre autores

T. Frolova

Papanin Institute for Biology of Inland Waters

Autor responsável pela correspondência
Email: bianka28061981@gmail.com
Russia, Yaroslavl Region, Borok

G. Izvekova

Papanin Institute for Biology of Inland Waters

Email: bianka28061981@gmail.com
Russia, Yaroslavl Region, Borok

Bibliografia

  1. Scholz T, Kuchta R, Oros M (2021) Tapeworms as pathogens of fish: A review. J Fish Diseases. 44: 1883–1900. https://doi.org/10.1111/jfd.13526
  2. Kuperman BI (1981) Tapeworms of the genus Trienophorus, parasites of fishes. New Delhi: Amerind Publishing Co. Pvt. Ltd.
  3. Pappas PW, Narcisi EM, Rentko V (1983) Alteration in brush border membrane proteins and membrane-bound enzymes of the tapeworm Hymenolepis diminuta during development in the definitive host. Mol Bioch Parasitol 8: 317–323.
  4. Cwiklinski K, Dalton JP (2018) Advances in Fasciola hepatica research using ‘omics’ technologies. Int J Parasitol 48: 321–331. https://doi.org/10.1016/j.ijpara.2017.12.001
  5. Zhang Y, Guo J, He L, Zong H-Y, Cai G-B (2018) Isolation and characterization of a novel serine protease inhibitor, SjSPI, from Schistosoma japonicum. Parasitol Int 67:415–424. https://doi.org/10.1016/j.parint.2018.04.002
  6. Zang X Maizels RM (2001) Serine proteinase inhibitors from nematodes and the arms race between host and pathogen. TRENDS Biochem Sci 26: 191–197.
  7. Morris SR, Sakanari JA (1994) Characterization of the serine protease and serine protease inhibitor from the tissue-penetrating nematode Anisakis simplex. J Biol Chem 269: 27650–27656.
  8. Kutyrev IA, Mazur OE, Goreva OB, Mordvinov VA (2020) A study of protein fractional composition during incubation of Diphyllobothrium dendriticum (Cestoda) plerocercoids in a medium containing blood serum of the host, the baikal omul Coregonus migratorius (Coregonidae). Biol Bull 47: 490–496. https://doi.org/10.1134/S1062359020040081
  9. Izvekova GI, Frolova TV, Izvekov EI (2017) Adsorption and inactivation of proteolytic enzymes by Triaenophorus nodulosus (Cestoda). Helminthologia 54: 3–10. https://doi.org/10.1515/helm-2017-0001
  10. Rogozhin E, Solovyev M, Frolova T, Izvekova G (2019) Isolation and partial structural characterization of new Kunitz-type trypsin inhibitors from the pike cestode Triaenophorus nodulosus. Mol Biochem Parasitol 233: 111217. https://doi.org/10.1016/j.molbiopara.2019.111217
  11. Oaks J, Knowles W, Cain G (1977) A simple method of obtaining an enriched fraction of tegumental brush border from Hymenolepis diminuta. J Parasitol 63: 476–485.
  12. Bradford MM (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Analytical Biochem 72: 248–254.
  13. Alarcón, FJ, Martínez TF, Barranco P, Cabello T, Díaz M, Moyano FJ (2002) Digestive proteases during development of larvae of red palm weevil, Rhynchophorus errugineus (Olivier, 1790) (Coleoptera: Curculionidae). Insect Biochem Mol Biol 32: 265–274.
  14. Nolasco-Soria H (2021) Improving and standardizing protocols for alkaline protease quantification in fish. Rev Aquacult 13: 43–65. https://doi.org/10.1111/raq.12463
  15. Holm H, Hanssen LE, Krogdahl A, Florholmen J (1988) High and low inhibitor soybean meals affect human duodenal proteinase activity differently: in vivo comparison with bovine serum albumin. J Nutrit 118: 515–520.
  16. Worthington Biochemical Corporation, 1991. Worthington Enzyme Manual: Enzymes, Enzyme Reagents. Freehold, N.J.
  17. Zimic MJ, Infantes J, López C, Velásquez J, Farfán M, Pajuelo M, Sheen P, Verastegui M, Gonzalez A, García HH, Gilman RH (2007) Comparison of the peptidase activity in the oncosphere excretory/secretory products of Taenia solium and Taenia saginata. J Parasitol 93: 727–734.
  18. Пронина СВ, Пронин НМ (1988) Взаимоотношения в системах гельминты–рыбы. М. Наука. [Pronina SV, Pronin NM (1988) Relationships in helminth–fish systems. M. Nauka (In Russ)].
  19. Izvekova GI, Tyutin AV (2014) Activity of digestive enzymes and distribution of the trematode Bunodera luciopercae (Muller) in the intestine of juvenile perch infected with plerocercoids of Triaenophorus nodulosus (Pallas). Inland Water Biol 7:167–171. https://doi.org/10.1134/S1995082914010076
  20. Frolova TF, Parshukov AN, Izvekova GI (2018) Activity of digestive enzymes in perch infected with Triaenophorus nodulosus (Pallas) plerocercoids. Inland Water Biol 11:501–506. https://doi.org/10.1134/S1995082918040053
  21. Kochneva A, Drozdova P, Borvinskaya E (2020). The first transcriptomic resource for the flatworm Triaenophorus nodulosus (Cestoda: Bothriocephalidea), a common parasite of holarctic freshwater fish. Marine Genomics 51: 100702. https://doi.org/10.1016/j.margen.2019.100702
  22. Borvinskaya E, Kochneva A, Bedulina D, Sukhovskaya I, Smirnov L, Babkina I (2021) Comparative Analysis of Proteins of Functionally Different Body Parts of the Fish Parasites Triaenophorus nodulosus and Triaenophorus crassus. Acta Parasitol 66: 1137–1150. https://doi.org/10.1007/s11686-021-00384-6
  23. de Magalhaes MTQ, Mambelli FS, Santos BPO, Morais SB, Oliveira SC (2018) Serine protease inhibitors containing a Kunitz domain: their role in modulation of host inflammatory responses and parasite survival. Microb Infect 20: 606–609. https://doi.org/10.1016/j.micinf.2018.01.003
  24. Izvekova GI, Frolova TV, Izvekov EI, Kashinskaya EN, Solovyev MM (2021) Localization of the proteinase inhibitor activity in the fish cestode Eubothrium rugosum. J Fish Dis 44: 1951–1958. https://doi.org/10.1111/jfd.13508
  25. Kashinskaya EN, Simonov EP, Poddubnaya LG, Vlasenko PG, Shokurova AV, Parshukov AN, Andree KB, Solovyev MM (2023) Trophic diversification and parasitic invasion as ecological niche modulators for gut microbiota of whitefish. Front Microbiol 14: 1090899. https://doi.org/10.3389/fmicb.2023.1090899
  26. Lombardo JF, Pórfido JL, Sisti MS, Giorello AN, Rodríguez S, Córsico B,·Franchini GR (2022) Function of lipid binding proteins of parasitic helminths: still a long road. Parasitol Res 121: 1117–1129. https://doi.org/10.1007/s00436-022-07463-1
  27. Xu J, Wu L, Sun Y, Wei Y, Zheng L, Zhang J, Pang Z, Yang Y, Lu Y (2020) Proteomics and bioinformatics analysis of Fasciola hepatica somatic proteome in different growth phases. Parasitol Res 119: 2837–2850. https://doi.org/10.1007/s00436-020-06833-x
  28. Victor B, Kanobana K, Gabriël S, Polman K, Deckers N, Dorny P, Deelder AM, Palmblad M (2012) Proteomic analysis of Taenia solium metacestode excretion-secretion proteins. Proteomics 11: 1860–1869.

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Declaração de direitos autorais © Т.В. Фролова, Г.И. Извекова, 2023

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