Benzofuran Spiropyrans as Analytical Reagents for Low-Molecular-Weight Aminothiols

A. A. Shcherbatykh; Щербатых А. А.; O. I. Askalepova; Аскалепова О. И.; A. O. Bulanov; Буланов А. О.; I. N. Shcherbakov; Щербаков И. Н.; S. T. Nguyen; Нгуен С. Т.

doi:10.31857/S0044450223010127

Benzofuran Spiropyrans as Analytical Reagents for Low-Molecular-Weight Aminothiols

Autores: Shcherbatykh A.¹, Askalepova O.¹, Bulanov A.¹, Shcherbakov I.¹, Nguyen S.¹
Afiliações:
1. Department of Chemistry, Southern Federal University
Edição: Volume 78, Nº 2 (2023)
Páginas: 151-158
Seção: ОРИГИНАЛЬНЫЕ СТАТЬИ
URL: https://journals.rcsi.science/0044-4502/article/view/136007
DOI: https://doi.org/10.31857/S0044450223010127
EDN: https://elibrary.ru/CEFYIX
ID: 136007

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Resumo

The problem of determining biologically active low-molecular-weight aminothiols, such as cysteine and glutathione, requires the development of new methods and analytical reagents. Among the latter substances, a group of spiropyrans can be distinguished, which are photochromic organic substances reaching an equilibrium between their cyclic and open forms in solutions. We consider a possibility of using benzofuran spiropyrans as analytical reagents for aminothiols and studied the effect of various substituents in their structure on the change in optical properties in the presence of cysteine and glutathione. An equilibrium in solutions of benzofuran spiropyrans bearing a hydroxyl group in position 7 shifts in the presence of analytes towards the formation of a colored open form. The considered spiropyrans were used to create a simple, selective, and sensitive procedure for the spectrophotometric and kinetic determination of cysteine and glutathione in vitro.

Palavras-chave

cysteine, glutathione, aminothiols, spiropyrans

Bibliografia

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