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DETERMINATION OF FORMALDEHYDE, GLYOXAL, GLUTARALDEHYDE, AND o-PHTHALALDEHYDE IN THE PRESENCE OF EACH OTHER IN DISINFECTANTS USING 2,4-DINTROPHENYLHYDRAZINE
- Authors: Salimova A.D1, Andreev S.V1,2, Martynov L.Y.2, Ischenco A.A2, Solovov R.D1,2
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Affiliations:
- Institute of Disinfectology of the Federal Scientific Center of Hygiene named after F. F. Erisman of the Federal Service for Surveillance on Consumer Rights Protection and Human Wellbeing
- Lomonosov Institute of Fine Chemical Technologies, MIREA – Russian Technological University
- Issue: Vol 80, No 12 (2025)
- Pages: 1311-1318
- Section: ORIGINAL ARTICLES
- Submitted: 03.12.2025
- URL: https://journals.rcsi.science/0044-4502/article/view/355765
- DOI: https://doi.org/10.7868/S3034512X25120032
- ID: 355765
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Abstract
Dialdehydes are predominantly used as disinfectants because these compounds exhibit a broad spectrum of antimicrobial activity. Among monoaldehydes, formaldehyde is used to a limited extent due to its higher toxicity. This work describes conditions for the simultaneous derivatization of formaldehyde, glyoxal, glutaraldehyde, and o-phthalaldehyde with 2,4-dinitrophenylhydrazine. The reaction is carried out in an acetonitrile–methanol mixture at 50°C in an ultrasonic bath using trifluoroacetic acid as a catalyst. The best separation of mixture components was achieved on a C18 column in gradient elution mode with acetonitrile and acetate buffer solution (pH 5.4) at a variable flow rate. The linearity range for formaldehyde was 2.51–20.0 mg/L, for glutaraldehyde 4.92–21.9 mg/L, for o-phthalaldehyde 1.98–6.94 mg/L, and for glyoxal 2.00–10.0 mg/L. The limits of detection for formaldehyde, glyoxal, glutaraldehyde, and o-phthalaldehyde were 0.453, 0.177, 0.967, and 0.760 mg/L, respectively. The developed method was successfully applied for the simultaneous determination of aldehydes in disinfectants.
About the authors
A. D Salimova
Institute of Disinfectology of the Federal Scientific Center of Hygiene named after F. F. Erisman of the Federal Service for Surveillance on Consumer Rights Protection and Human WellbeingMoscow, Russia
S. V Andreev
Institute of Disinfectology of the Federal Scientific Center of Hygiene named after F. F. Erisman of the Federal Service for Surveillance on Consumer Rights Protection and Human Wellbeing; Lomonosov Institute of Fine Chemical Technologies, MIREA – Russian Technological University
Email: svandreev.niid@gmail.com
Moscow, Russia; Moscow, Russia
L. Yu Martynov
Lomonosov Institute of Fine Chemical Technologies, MIREA – Russian Technological UniversityMoscow, Russia
A. A Ischenco
Lomonosov Institute of Fine Chemical Technologies, MIREA – Russian Technological UniversityMoscow, Russia
R. D Solovov
Institute of Disinfectology of the Federal Scientific Center of Hygiene named after F. F. Erisman of the Federal Service for Surveillance on Consumer Rights Protection and Human Wellbeing; Lomonosov Institute of Fine Chemical Technologies, MIREA – Russian Technological UniversityMoscow, Russia; Moscow, Russia
References
- Jones S., Reagan K., Saunders N. Antiseptics, disinfectants, and sterilization / Advanced Monitoring and Procedures for Small Animal Emergency and Critical Care. Wiley, 2023. p. 837.
- Al Shikh A., Milosevic A. Effectiveness of alcohol and aldehyde spray disinfectants on dental impressions // Clin. Cosmet. Investig. Dent. 2020. V. 12, P. 25. https://doi.org/10.2147/CCIDE.S233336
- Frost L., Tully M., Dixon L., Hicks H.M., Bennett J., Stokes I., Marsella L., Gubbins S., Batten C. Evaluation of the efficacy of commercial disinfectants against African swine fever virus // Pathogens. 2023. V. 12. P. 855. https://doi.org/10.3390/pathogens12070855
- David V., Moldoveanu S.C., Galoon T. Derivatization procedures and their analytical performances for HPLC determination in bioanalysis // Biomed. Chromatogr. 2021. V. 35. Article e5008. https://doi.org/10.1002/bmc.5008
- Donegatti T.A., Lobato A., Moreira Gonçalves L., Alves Pereira E. Cyclohexane-1,3-dione as a derivatizing agent for the analysis of aldehydes by micellar electrokinetic chromatography with diode array detection // Electrophoresis. 2019. V. 40. P. 2929. https://doi.org/10.1002/elps.201900171
- Lu Y., Yao D., Chen C. 2-Hydrazinoquinoline as a Derivatization agent for LC-MS-based metabolomic investigation of diabetic ketoacidosis // Metabolites. 2013. V. 3. P. 993. https://doi.org/10.3390/metabo3040993
- Elias R.J., Laurie V.F., Ebeler S.E., Wong J.W., Waterhouse A.L. Analysis of selected carbonyl oxidation products in wine by liquid chromatography with diode array detection // Anal. Chim. Acta. 2008. V. 626. P. 104. https://doi.org/10.1016/j.aca.2008.07.048
- Douny C., Tihon A., Bayonnet P., Brose F., Degand G., Rozet E., Milet J., Ribonnet L., Lambin L., Larondelle Y., Scippo M.-L. Validation of the analytical procedure for the determination of malondialdehyde and three other aldehydes in vegetable oil using liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS) and application to linseed oil // Food Anal. Methods. 2015. V. 8. P. 1425. https://doi.org/10.1007/s12161-014-0028-z
- Basheer C., Pawagadhi S., Yu H., Balasubramanian R., Lee H.K. Determination of aldehydes in rainwater using micro-solid-phase extraction and high-performance liquid chromatography // J. Chromatogr. A. 2010. V. 1217. P. 6366. https://doi.org/10.1016/j.chroma.2010.08.012
- Ma L., Liu G. Simultaneous analysis of malondialdehyde, 4-hydroxy-2-hexenal, and 4-hydroxy-2-nonenal in vegetable oil by reversed-phase high-performance liquid chromatography // J. Agric. Food Chem. 2017. V. 65. P. 11320. https://doi.org/10.1021/acs.jafc.7b04566
- Kishikawa N., El-Maghrabey M.H., Kuroda N. Chromatographic methods and sample pretreatment techniques for aldehydes determination in biological, food, and environmental samples // J. Pharm. Biomed. Anal. 2019. V. 175. Article 112782. https://doi.org/10.1016/j.jpba.2019.112782
- Barnes A.R. Determination of glutaraldehyde in solution as its bis-2,4-dinitrophenylhydrazone derivative; determination of geometrical isomer ratios // Pharm. Acta Helv. 1993. V. 68. P. 113. https://doi.org/10.1016/0031-6865(93)90013-V
- Thanh N.H., Lan D.T.N., Ha P.T.T., An V.T.T., Khanh C.C. High performance liquid chromatography analytical method for glutaraldehyde determination in disinfectants // Vietnam J. Food Control. 2022. V. 5. P. 160.
- El-Maghrabey M., Suzuki H., Kishikawa N., Kuroda N. A sensitive chemiluminescence detection approach for determination of 2,4-dinitrophenylhydrazine derivatized aldehydes using online UV irradiation – luminol CL reaction. Application to the HPLC analysis of aldehydes in oil samples // Talanta. 2021. V. 233. Article 122522. https://doi.org/10.1016/j.talanta.2021.122522
- Binding N. Simultaneous determination of airborne acetaldehyde, acetone, 2-butanone, and cyclohexanone using sampling tubes with 2,4-dinitrophenylhydrazine-coated solid sorbent // Toxicol. Lett. 1998. V. 96–97. P. 289. https://doi.org/10.1016/S0378-4274(98)00085-X
- Doronin S.Y., Chernova R.K., Burmistrova A.A. Effect of the micellar surfactant nanoreactors on the reactions of 2,4-dinitrophenylhydrazine with some aldehydes // Russ. J. Gen. Chem. 2008. V. 78. P. 903. https://doi.org/10.1134/S1070363208050113
- Magnusson B., Örnemark U. Eurachem Guide: The Fitness for Purpose of Analytical Methods – A Laboratory Guide to Method Validation and Related Topics. 2nd ed. Eurachem, 2014. 70 p.
- Borman P., Elder D. Q2 (R1) validation of analytical procedures: Text and methodology. ICH Quality Guidelines: an Implementation Guide. 2017. p. 127.
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