Using a Magnetic Sorbent Based on Hypercrosslinked Polystyrene in Combination with Gas Chromatography–Mass Spectrometry for the Determination of Chlorophenols in Freshwater Fish

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Abstract

A combination of solid-phase extraction on a magnetic sorbent based on hypercrosslinked polystyrene and gas chromatography–mass spectrometry is proposed for the determination of chlorophenols in freshwater fish. I2-Chlorophenol, 3-chlorophenol, 4-chlorophenol, 2,4-dichlorophenol, 2,4,6-trichlorophenol, and pentachlorophenol were identified in samples of roach (Rutilus rutilus) and silver carp (Hypophthalmichthys molitrix). The accumulation of chlorophenols in various organs and tissues of fish (muscles, liver, milk, caviar, body fat) living in the Voronezh reservoir was studied. Methods for sample preparation were developed and the interfering influences of various factors on the determination results were found. Oil products and fatty acids make the greatest contribution to matrix effects. The limits of the determination of chlorophenols by the developed method are 7–18 ng/kg in muscle tissue and liver and 30–60 ng/kg in fat-containing tissues. The maximum amounts of chlorophenols were found in the fat deposits of silver carp and amounted to 100, 82, 434, 1529, 383 and 411 ng/kg for 2-chlorophenol, 3-chlorophenol, 4-chlorophenol, 2,4-dichlorophenol, 2,4,6-trichlorophenol, and pentachlorophenol, respectively.

About the authors

A. S. Gubin

Voronezh State University of Engineering Technologies

Email: goubinne@mail.ru
394036, Voronezh, Russia

P. T. Sukhanov

Voronezh State University of Engineering Technologies

Email: goubinne@mail.ru
394036, Voronezh, Russia

A. A. Kushnir

Voronezh State University of Engineering Technologies

Author for correspondence.
Email: goubinne@mail.ru
394036, Voronezh, Russia

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