Effect of Glycodelin on the Level of T-Regulatory Lymphocytes and Acute Phase Proteins in Wistar Rats after Introduction of Allogenous Bone Marrow Cells

S. A. Zamorina; Заморина С. А.; M. S. Bochkova; Бочкова М. С.; V. P. Timganova; Тимганова В. П.; S. V. Uzhviyuk; Ужвиюк С. В.; K. Yu. Shardina; Шардина К. Ю.; V. V. Vlasova; Власова В. В.; M. B. Rayev; Раев М. Б.

doi:10.31857/S0041377123050103

Effect of Glycodelin on the Level of T-Regulatory Lymphocytes and Acute Phase Proteins in Wistar Rats after Introduction of Allogenous Bone Marrow Cells

Authors: Zamorina S.A.¹^,2, Bochkova M.S.¹^,2, Timganova V.P.¹, Uzhviyuk S.V.¹, Shardina K.Y.¹, Vlasova V.V.¹, Rayev M.B.¹^,2
Affiliations:
1. Institute of Ecology and Genetics of Microorganisms, Ural Branch of the Russian Academy of Sciences (“IEGM UB RAS”) – a branch of the Federal State Budgetary Institution of the Perm Federal Research Center of the Ural Branch of the Russian Academy of Sciences
2. Perm State University, Biology Faculty
Issue: Vol 65, No 5 (2023)
Pages: 475-482
Section: Articles
URL: https://journals.rcsi.science/0041-3771/article/view/140101
DOI: https://doi.org/10.31857/S0041377123050103
EDN: https://elibrary.ru/DGDGMF
ID: 140101

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Abstract
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Abstract

Amniotic variant of glycodelin (GdA) has pronounced immunomodulatory properties, participating in the formation of immune tolerance during pregnancy. We investigated the effect of glycodelin on the level of T regulatory lymphocytes (Treg) and the level of acute phase proteins (α-2-macroglobulin (α-2M), orosomucoid, C-reactive protein (CRP)) upon administration of allogeneic red bone marrow (BM) cells to Wistar rats in a dynamic experiment in vivo. It was found that the introduction of GdA in animal whith allogeneic BM led to an increase in the proportion of peripheral Treg among CD4⁺ lymphocytes at the end of the experiment (on the 21st day) in comparison with the group that was injected with BM. It was shown that glycodelin reduced the level of CRP and α-2M, but increased the level of orosomucoid in the serum of experimental animals at the beginning of the experiment (day 3), however, by the end of the experiment (day 21), normalization of protein values was observed in all groups of experimental animals acute phase to the level of intact animals. Thus, glycodelin is able to realize an immunosuppressive effect on allogeneic cells through an increase in the level of Treg and orosomucoid, as well as a decrease in the concentration of CRP and α-2M.

Keywords

bone marrow, transplantation, rats, glycodelin, regulatory T lymphocytes, acute inflammation phase proteins

About the authors

S. A. Zamorina

Institute of Ecology and Genetics of Microorganisms, Ural Branch of the Russian Academy of Sciences (“IEGM UB RAS”) – a branch of the Federal State Budgetary Institution of the Perm Federal Research Center of the Ural Branch of the Russian Academy of Sciences; Perm State University, Biology Faculty

Author for correspondence.
Email: zamorina.sa@gmail.com
Russian Federation, 614081, Perm; Russian Federation, 614068, Perm

References

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