Response of the Endometrial Mesenchymal Stem Cell Genome to the Procedure of Long-Term Cryopreservation

T. M. Grinchuk; Гринчук Т. М.; M. A. Shorokhova; Шорохова М. А.; N. A. Pugovkina; Пуговкина Н. А.

doi:10.31857/S0041377123040065

Response of the Endometrial Mesenchymal Stem Cell Genome to the Procedure of Long-Term Cryopreservation

Authors: Grinchuk T.M.¹, Shorokhova M.A.¹, Pugovkina N.A.¹
Affiliations:
1. Institute of Cytology, Russian Academy of Sciences
Issue: Vol 65, No 4 (2023)
Pages: 354-366
Section: Articles
URL: https://journals.rcsi.science/0041-3771/article/view/140074
DOI: https://doi.org/10.31857/S0041377123040065
EDN: https://elibrary.ru/ZGVVAN
ID: 140074

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Abstract
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Abstract

Information about the effect of cryopreservation on functions and the genetic of cells of different genesis is not unambiguous and is in the process of accumulation. This work is aimed at studying the effect of long-term storage (7 years) of human endometrial mesenchymal stem cells (eMSCs) in the frozen state on the stability of their genome in vitro. The results showed destabilization of the karyotype structure in the descendants of cells after their thawing, namely, aneupolyploidization, increased fragility of chromosomes, resulting in a huge pool of aberrant chromosomes, and impaired condensation in homologues. Chromosomal breakds in centromeric regions offen accompanied by the preservation of genetic material in the form of independent chromosomes. Almost all chromosomes of the set were involved in the process of destabilization of the eMSCs cell genome. It has been shown that the procedure of long-term cryopreservation can become an inducer of premature cellular aging of eMSCs after their thawing. Comparison of the data obtained with the results of karyotyping of transformed Chinese hamster cells that underwent a similar procedure led to the conclusion that cryopreservation for biological systems can be a stress that induces genetic defects of various types at the karyotype level. The response of the genome of cells of different origin to the same conditions of cryopreservation may differ.

Keywords

human endometrial mesenchymal stem cells, karyotype, chromosomes, cryogenic freezing, genome instability

References

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