Hydrogen effect on the mechanisms of mucosal immunity in patients with COVID-19
- 作者: Svitich O.1, Baranova I.2, Kryukova N.2, Poddubikov A.1, Vinnitskaya A.1, Abramova N.1, Zakharova V.2, Shogenova L.2, Kostinov M.1, Chuchalin A.2
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隶属关系:
- Mechnikov Research Institute for Vaccines and Serums
- Pirogov Russian National Research Medical University
- 期: 卷 94, 编号 3 (2022)
- 页面: 372-377
- 栏目: Original articles
- URL: https://journals.rcsi.science/0040-3660/article/view/89469
- DOI: https://doi.org/10.26442/00403660.2022.03.201398
- ID: 89469
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Aim. To study the inhalation of an active form of hydrogen effect to mucosal and system immunity in a rehabilitation program for health workers.
Materials and methods. The study involved patients that survived COVID-19 after therapy with inhaled hydrogen for 90 minutes (n=30), and a control group of patients treated according to standard protocol for managing patients that survived COVID-19 during the rehabilitation period (n=30). Biomaterial was carried out in 2 stages: on the first day of the study, before the accepted therapy and on the 10th day of the study. The indicators of humoral and cellular immunity were studied. The levels of secretory immunoglobulin A (sIgA) and IgG were investigated using the method of enzyme-linked immunosorbent assay. Phagocytosis was assessed on a Beckman Coulter FC-500 flow cytometer. Statistical data processing was carried out in the GraphPad Prism 7.00 software using nonparametric methods.
Results. It was shown that the phagocytic index (PI) of monocytes in nasal scrapings after inhaled hydrogen treatment did not significantly change relative to the first day of treatment and control, while the PI of granulocytes in nasal scrapings significantly increased relative to the first day by 2.5 times (p=0.000189), as well as relative to the control by 1.1 times (p=0.047410). PI of monocytes in pharyngeal scrapings showed a significant increase relative to the first day of treatment by 2.8 times (p=0.041103), however, did not differ relative to the control. PI of granulocytes of pharyngeal scraping did not differ significantly relative to the first day and control. PI of granulocytes and blood monocytes of the studied group did not change significantly. PI of granulocytes and monocytes of peripheral blood relative to control during therapy did not change. The sIgA level in nasal scrapings significantly increased by 2.9 times, while in pharyngeal scrapings the level of sIgA significantly decreased by 2 times.
Сonclusion. We have shown an increase in granulocytes PI in the nasal cavity and oral monocytes, as well as in the level of sIgA in the nasal cavity during therapy with active hydrogen. The data obtained indicate the effectiveness of therapy, which can be used both in the treatment of COVID-19, and in post-COVID syndrome as an additional therapy. The absence of changes in blood parameters, as well as individual links in nasal and pharyngeal scrapings, requires further study to develop ways to overcome treatment tolerance.
作者简介
Oxana Svitich
Mechnikov Research Institute for Vaccines and Serums
编辑信件的主要联系方式.
Email: svitichoa@yandex.ru
ORCID iD: 0000-0003-1757-8389
SPIN 代码: 8802-5569
чл.-кор. РАН, д-р мед. наук, проф., дир., зав. лаб. молекулярной иммунологии
俄罗斯联邦, MoscowIrina Baranova
Pirogov Russian National Research Medical University
Email: svitichoa@yandex.ru
ORCID iD: 0000-0002-2469-7346
д-р мед. наук, проф., проф. каф. госпитальной терапии педиатрического фак-та
俄罗斯联邦, MoscowNadezhda Kryukova
Pirogov Russian National Research Medical University
Email: svitichoa@yandex.ru
ORCID iD: 0000-0002-8167-0959
аспирант, ассистент каф. госпитальной терапии педиатрического фак-та
俄罗斯联邦, MoscowAlexander Poddubikov
Mechnikov Research Institute for Vaccines and Serums
Email: svitichoa@yandex.ru
ORCID iD: 0000-0001-8962-4765
канд. мед. наук, зав. лаб. микробиологии условно-патогенных бактерий
俄罗斯联邦, MoscowAlexandra Vinnitskaya
Mechnikov Research Institute for Vaccines and Serums
Email: svitichoa@yandex.ru
ORCID iD: 0000-0003-0717-8648
науч. сотр. лаб. молекулярной иммунологии
俄罗斯联邦, MoscowNatalia Abramova
Mechnikov Research Institute for Vaccines and Serums
Email: svitichoa@yandex.ru
ORCID iD: 0000-0002-7307-0515
мл. науч. сотр. лаб. молекулярной иммунологии
俄罗斯联邦, MoscowValeria Zakharova
Pirogov Russian National Research Medical University
Email: svitichoa@yandex.ru
ORCID iD: 0000-0003-3941-1934
ординатор каф. госпитальной терапии педиатрического фак-та
俄罗斯联邦, MoscowLudmila Shogenova
Pirogov Russian National Research Medical University
Email: svitichoa@yandex.ru
ORCID iD: 0000-0001-9285-9303
канд. мед. наук, доц. каф. внутренних болезней педиатрического фак-та
俄罗斯联邦, Moscow
Mikhail Kostinov
Mechnikov Research Institute for Vaccines and Serums
Email: svitichoa@yandex.ru
ORCID iD: 0000-0002-1382-9403
д-р мед. наук, проф., зав. лаб. вакцинопрофилактики и иммунотерапии аллергических заболеваний
俄罗斯联邦, MoscowAlexander Chuchalin
Pirogov Russian National Research Medical University
Email: svitichoa@yandex.ru
ORCID iD: 0000-0002-5070-5450
акад. РАН, д-р мед. наук., проф., зав. каф. госпитальной терапии педиатрического фак-та, председатель правления Российского респираторного общества
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