IMMUNOLOGICAL DISORDERS AND CYTOKINE PROFILE IN SYSTEMIC AND LOCALIZED SCLEROSIS

Aim. Comparative study of disorders in cellular immunity and cytokine profile in systemic and localized sclerosis (SS, LS). Material and methods. Parameters of cellular immunity and cytokine profile were examined in 58 systemic sclerosis patients (24 patients with SS, 34 patients with LS and 33 healthy donors). The examination protocol included assay of circulating immune complexes (CIC), serum cytokines (IL-1beta, IL-4, IL-6, TNF), DNA antibodies, assessment of oxygen-dependent and non-oxygen-dependent functions of circulating neutrophils and monocytes, identification of lymphocytic phenotype. Results. Elevated levels of CIC, proinflammatory cytokines in SS and LS, antibodies to native doublechain DNA in LS were found. Free radical oxidation of neutrophils in LS was higher only by one parameter, in SS by all spontaneous tests of neutrophils and monocytes. Non-oxygen-dependent metabolism of phagocytes was enhanced only in SS. Patients with LS and SS have subnormal count of lymphocytes CD3+, CD4+, CD11b+ in the presence of elevated count of lymphocytesCD8+, CD16+ as well as activation markers — CD19+, CD25+, CD95+ and HLA-DR+ cells. CD25+ lymphocytes count is higher in SS than in LS. Conclusion. The study of metabolism of circulating phagocytes, cytokine profile lymphocytic phenotype can be used for differential diagnosis of LS with SS.

localized and systemic sclerosis, immunological tests