Adiponectin gene expression in local fat depots in patients with coronary heart disease depending on the degree of coronary lesion

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Abstract

Aim. To determine the dependence of adiponectin gene expression by subcutaneous, epicardial and perivascular adipocytes on the degree of coronary lesion in coronary heart disease.

Materials and methods. 84 patients with coronary artery disease were examined. Of these, 39 people showed a moderate degree of atherosclerotic lesion of the coronary bed (less than or equal to 22 points) on the SYNTAX Score scale, 20 – severe (22–31 points), and 25 – extremely severe (more than 32 points). Upon admission to the hospital, all patients underwent an echocardiographic study (Echocardiography, Acuson, Germany) with the calculation of the ejection fraction (EF) of the left ventricle (LV) to assess its systolic function. During a planned surgical intervention (coronary bypass surgery, CABG), adipocytes of subcutaneous, epicardial (EAT) and perivascular adipose tissue (PVAT) were taken. Adiponectin gene expression was evaluated by polymerase chain reaction (real-time PCR) using TaqMan probes. Statistical analysis was performed using Statistica 9.0.

Results. The maximum level of adiponectin expression was detected in adipocytes of PVAT, and the minimum – EAT. With an increase in the degree of atherosclerotic lesion of the coronary bed, the expression of the adiponectin gene in adipocytes of local depots significantly decreases r=-0.82; p=0.023. Moreover, the low level of gene expression in EAT correlated with a decrease in LV EF by r=0.73; p=0.03. In adipocytes of subcutaneous and especially PVAT, gene expression was the highest in patients with a moderate degree of coronary lesion.

Conclusions. Low adiponectin gene expression in EAT is associated with an increase in the degree of atherosclerotic lesion of the coronary bed and a decrease in LV EF.

About the authors

E. V. Belik

Research Institute for Complex Issues of Cardiovascular Disease

Author for correspondence.
Email: sionina.ev@mail.ru
ORCID iD: 0000-0003-3996-3325

мл.н.с. лаб. исследований гомеостаза отд. диагностики сердечно-сосудистых заболеваний

Russian Federation, Kemerovo

O. V. Gruzdeva

Research Institute for Complex Issues of Cardiovascular Disease

Email: sionina.ev@mail.ru
ORCID iD: 0000-0002-7780-829X

д.м.н., зав. лаб. исследований гомеостаза отд. диагностики сердечно-сосудистых заболеваний

Russian Federation, Kemerovo

O. E. Akbasheva

Siberian State Medical University

Email: sionina.ev@mail.ru
ORCID iD: 0000-0003-0680-8249

д.м.н., проф. каф. биохимии и молекулярной биологии с курсом клинической лабораторной диагностики, заместитель декана медико-биологического фак-та

Russian Federation, Tomsk

Yu. A. Dyleva

Research Institute for Complex Issues of Cardiovascular Disease

Email: sionina.ev@mail.ru
ORCID iD: 0000-0002-6890-3287

к.м.н., с.н.с. лаб. исследований гомеостаза отд. диагностики сердечно-сосудистых заболеваний

Russian Federation, Kemerovo

D. A. Borodkina

Research Institute for Complex Issues of Cardiovascular Disease

Email: sionina.ev@mail.ru
ORCID iD: 0000-0002-6221-3509

к.м.н., с.н.с. лаб. исследований гомеостаза отд. диагностики сердечно-сосудистых заболеваний

Russian Federation, Kemerovo

M. Yu. Sinitsky

Research Institute for Complex Issues of Cardiovascular Disease

Email: sionina.ev@mail.ru
ORCID iD: 0000-0002-4824-2418

к.б.н., с.н.с. лаб. геномной медицины отд. экспериментальной и клинической кардиологии

Russian Federation, Kemerovo

A. V. Sotnikov

Research Institute for Complex Issues of Cardiovascular Disease

Email: sionina.ev@mail.ru
ORCID iD: 0000-0002-1828-5652

врач сердечно-сосудистый хирург кардиохирургического отд-ния

Russian Federation, Kemerovo

K. A. Kozyrin

Research Institute for Complex Issues of Cardiovascular Disease

Email: sionina.ev@mail.ru

врач сердечно-сосудистый хирург кардиохирургического отд-ния

Russian Federation, Kemerovo

N. K. Brel

Research Institute for Complex Issues of Cardiovascular Disease

Email: sionina.ev@mail.ru
ORCID iD: 0000-0002-5643-4022

врач-рентгенолог отд-ния лучевой диагностики

Russian Federation, Kemerovo

D. Yu. Naumov

Research Institute for Complex Issues of Cardiovascular Disease

Email: sionina.ev@mail.ru

лаборант-исследователь лаб. интервенционных методов диагностики и лечения

Russian Federation, Kemerovo

A. A. Shilov

Research Institute for Complex Issues of Cardiovascular Disease

Email: sionina.ev@mail.ru
ORCID iD: 0000-0002-4639-7416

к.м.н., с.н.с. лаб. интервенционных методов диагностики и лечения

Russian Federation, Kemerovo

E. E. Bychkova

Research Institute for Complex Issues of Cardiovascular Disease

Email: sionina.ev@mail.ru
ORCID iD: 0000-0002-0500-2449

лаборант-исследователь лаб. исследований гомеостаза отд. диагностики сердечно-сосудистых заболеваний

Russian Federation, Kemerovo

V. N. Karetnikova

Research Institute for Complex Issues of Cardiovascular Disease

Email: sionina.ev@mail.ru
ORCID iD: 0000-0002-9801-9839

д.м.н., проф., зав. лаб. патофизиологии мультифокального атеросклероза отд. мультифокального атеросклероза

Russian Federation, Kemerovo

O. L. Barbarash

Research Institute for Complex Issues of Cardiovascular Disease

Email: sionina.ev@mail.ru
ORCID iD: 0000-0002-4642-3610

чл.-кор. РАН, д.м.н., проф., дир.

Russian Federation, Kemerovo

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2. Fig. 1. Expression of the adiponectin gene in the culture medium of subcutaneous, epicardial and perivascular adipocytes in patients with coronary artery disease.

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3. Fig. 1. Expression of the adiponectin gene in the culture medium of subcutaneous, epicardial and perivascular adipocytes during primary isolation, depending on the degree of damage to the coronary bed.

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