Changes in gut microbiota with bronchial asthma

Aim. To study the intestinal microbiota changes in patients with bronchial asthma (BA).

Materials and methods. 40 patients and 15 healthy individuals were included for the study. The microbiota study in feces samples was performed by sequencing the 16SpRNA gene.

Results. It was noted an increasing of the Proteobacteria proportion in the patients with BA. The fractions of Betaproteobacteria и Gammaproteobacteria were increased in the patients with allergic BA and at the same time, only the Gammaproteobacteria part was increased in patients with non-allergic form of BA. It was found an increase in Bacilli and a decrease in the proportion bacteria forming butyrate (Anaerostipes, Faecalibacterium) and acetate (Alistipes), which was corresponded to a decrease in the proportion of strict anaerobic symbionts and an increase in the proportion of opportunistic facultative anaerobes. The relative bacteria amount was reduced for the Negativicutes Erysipelotrichia, Bacteroidia classes, the Erysipelotrichaceae, Pseudomonadaceae, Rhodospirillaceae, Bacillaceae families and for the kinds of Barnesiella, Paraprevotella, Pyrolobus, Bifidobacterium, Pseudomonas, Coprobacter, Bacillus in the allergic asthma patients with syndrome of intensive bacterial overgrowth (SIBO) cases. In the non-allergic asthma case, the presence of SIBO was accompanied by the relative bacteria amount increasing of the Bacteroidaceae and the Paraprevotella families and the Odoribacter, Bacteroides, Butyricicoccus, Parasutterella genera. The bacterial spectrum changes correlated with the main clinical and laboratory manifestations of BA in the patients.

Conclusion. The results have indicated the differences in the intestinal microflora composition of healthy volunteers and patients with bronchial asthma in including the SIBO presence. It is necessary more detail study of the bacterial composition changes in the intestine for the bronchopulmonary pathology case.