电邮这篇文章 Aberrant methylation of the promoter regions of the SOX7 and p15INK4b genes and Wnt signaling pathway antagonists in patients with acute myeloid leukemias
- 作者: Kostroma II1, Gritsaev SV1, Sidorova Z.Y.1, Tiranova SA1, Svitina SP1, Drizhun Y.S1, Chubukina Z.V1, Martynkevich IS1, Kapustin SI1, Bessmeltsev SS1
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隶属关系:
- Российский научно-исследовательский институт гематологии и трансфузиологии ФМБА
- 期: 卷 88, 编号 7 (2016)
- 页面: 31-36
- 栏目: Editorial
- URL: https://journals.rcsi.science/0040-3660/article/view/31989
- ID: 31989
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Aim. To investigate the methylation status of the SOX7 and p15NK4b genes and Wnt signaling pathway antagonists in patients with acute myeloid leukemia (AML) in order to assess the association of the rate of aberrant methylation (AM) with the morphological variant and pattern of chromosomal aberrations, as well as the impact of the methylation status on survival. Subjects and methods. The data of 57 AML patients aged 20 to 79 years were analyzed. The methylation status of the genes was studied by methylation-specific polymerase chain reaction. Results. The signs of the AM of ≥1 gene were detected in 52 (91.2%) of the 57 patients. The most common finding was AM of simultaneously 2 or 3 genes: in 29.8 and 21.1% of the patients, respectively. Concurrent methylation of 3-5 genes proved to be a more frequent finding in AML patients with myelodysplasia: in 7 (70%) of 10 patients. The proportion of patients with methylation of 5 genes was considerably higher in a group of patients with a complex karyotype: 50% versus 8.3% among other patients (odds ratio: 11.0; 95% confidence interval 2.0 to 61.6; p=0.01). There were no differences in the median overall and relapse-free survival rates in patients with a normal karyotype and without FLT3 and NPM mutations, who received induction therapy, in relation to the number of genes with AM. Conclusion. AM of the p15NK4b and SOX7 genes and Wnt signaling pathway antagonists is detected in the majority of patients with AML, which allows hypomethylating agents to be recommended for the treatment of patients who cannot use intensive cytostatic therapy for different reasons. The detection of a large number of genes with the aberrant methylation status in most AML patients with myelodysplasia or a complex karyotype serves as the basis for initiating trials to evaluate the efficiency of a combination of 5-azacytidine and cytostatics.
作者简介
I Kostroma
Российский научно-исследовательский институт гематологии и трансфузиологии ФМБАМосква, Россия
S Gritsaev
Российский научно-исследовательский институт гематологии и трансфузиологии ФМБАМосква, Россия
Zh Sidorova
Российский научно-исследовательский институт гематологии и трансфузиологии ФМБАМосква, Россия
S Tiranova
Российский научно-исследовательский институт гематологии и трансфузиологии ФМБАМосква, Россия
S Svitina
Российский научно-исследовательский институт гематологии и трансфузиологии ФМБАМосква, Россия
Yu Drizhun
Российский научно-исследовательский институт гематологии и трансфузиологии ФМБАМосква, Россия
Zh Chubukina
Российский научно-исследовательский институт гематологии и трансфузиологии ФМБАМосква, Россия
I Martynkevich
Российский научно-исследовательский институт гематологии и трансфузиологии ФМБАМосква, Россия
S Kapustin
Российский научно-исследовательский институт гематологии и трансфузиологии ФМБАМосква, Россия
S Bessmeltsev
Российский научно-исследовательский институт гематологии и трансфузиологии ФМБАМосква, Россия
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