Email this article COMMUNITY-ACQUIRED PNEUMONIA: COMPARISON OF ETIOLOGICAL DIAGNOSTIC METHODS IN CLINICAL PRACTICE
- Authors: Pustovalov AA1, Rvacheva AV1, Chuchalin AG2, Sokolov EI1, Khaptkhaeva GE2, Tkachev GA1, Zykov KA1
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Affiliations:
- Moscow State Medical Stomatological University
- Research Institute of Pulmonology
- Issue: Vol 84, No 4 (2012)
- Pages: 11-16
- Section: Editorial
- URL: https://journals.rcsi.science/0040-3660/article/view/31000
- ID: 31000
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Abstract
Aim. Comparison of different methods of community-acquired pneumonia (CAP) etiological diagnosis. Material and methods. A total of 20 male and 10 females patients 21 to 75 years of age entered the trial. All of them had CAP running a non-severe course. Microbiological and molecular-genetic methods were used to examine the patients’ sputum, blood serum serological tests were made to measure the level of IgM and IgG antibodies (AB) to Chlamydophila pneumoniae and Mycoplasma pneumoniae. Results. Pathogens were detected in 23(76.7%) patients. Microbiological examination (MBE) detected monobacterial contamination in 19 patients, polymerase chain reaction (pCR) identified monobacterial infection in 10 patients, mixed bacterial infection in 5 and viral-bacterial mixed contamination in 4 patients. MBE provided 19 positive results, PCR 30 ones. Serological examination detected IgM AB to Mycoplasma pneumoniae in 6 patients, sputum investigation with PCR detected the pathogen only in 2 patients. IgM AB to Chlamydophila pneumoniae were detected in 4 patients, PCR failed to detect infection agent. Conclusion. Current etiological diagnosis can be made with the highest efficacy with combination of three methods: microbiological for detection of bacterial pathogens (except atypical agents), molecular-genetic for identification of both bacterial and viral pathogens and serological for diagnosis of atypical pathogens.
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##article.viewOnOriginalSite##About the authors
A A Pustovalov
Moscow State Medical Stomatological Universityсоискатель каф. факультетской терапии и профессиональных болезней
A V Rvacheva
Moscow State Medical Stomatological University
Email: arvatcheva@mail.ru
канд. мед. наук, ст. науч. сотр. лаб. пульмонологии отд. клинической медицины НИМСИ МГМСУ
A G Chuchalin
Research Institute of Pulmonologyд-р мед. наук, проф., акад. РАМН, дир. НИИ пульмонологии
E I Sokolov
Moscow State Medical Stomatological Universityд-р мед. наук, проф., акад. РАМН, зав. каф. факультетской терапии
G E Khaptkhaeva
Research Institute of Pulmonologyаспирант
G A Tkachev
Moscow State Medical Stomatological Universityканд. мед. наук, мл. науч. сотр. лаб. пульмонологии отд. клинической медицины НИМСИ МГМСУ
K A Zykov
Moscow State Medical Stomatological Universityд-р мед. наук. зав. лаб. пульмонологии отд. клинической медицины НИМСИ МГМСУ
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