The study of parameters of mesenchymal stromal cells differentiation in donors and patients with aplastic anemia
- Authors: Svinareva D.A.1, Petrova T.V.1, Shipunova (Nifontova) I.N.1, Momotyuk K.S.1, Mikhaylova E.A.1, Drize N.I.1, Svinareva DA1, Petrova TV1, Shipunova (Nifontova) IN1, Momotyuk KS1, Mikhailova EA1, Drize NI1
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Affiliations:
- ГУ Гематологический научный центр РАМН
- Issue: Vol 81, No 7 (2009)
- Pages: 66-70
- Section: Editorial
- URL: https://journals.rcsi.science/0040-3660/article/view/30413
- ID: 30413
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Abstract
Material and methods. We obtained MSC cultures from BM cells of donors and AA patients and induced differentiation of mesenchymal cells with use of relevant reagents. Morphological changes in MSC were studied with light microscopy. A relative level of expression of differentiation marker genes in MSC cultures before and after induction of differentiation was analysed with reverse transcription-polymerase chain reaction.
Results. By morphological characteristics, MSC cultures in AA patients before and after differentiation induction do not differ from donor cultures, but relative expression of the genes of differentiation markers demonstrated that expression was different in male and female donors; MSC before and after induction of differentiation differ in donors and AA patients.
Conclusion. Further studies are needed for detection of functional changes in precursors of stromal microenvironment and understanding of the disease pathogenesis.
About the authors
Dar'ya Anatol'evna Svinareva
ГУ Гематологический научный центр РАМН
Email: svinareva@yandex.ru
лаборатория физиологии кроветворения, н. с; ГУ Гематологический научный центр РАМН
Tat'yana Vladimirovna Petrova
ГУ Гематологический научный центр РАМН
Email: petrozik@gmail.com
лаборатория физиологии кроветворения, аспирант; ГУ Гематологический научный центр РАМН
Irina Nikolaevna Shipunova (Nifontova)
ГУ Гематологический научный центр РАМН
Email: iranifontova@yandex.ru
лаборатория физиологии кроветворения, н. с; ГУ Гематологический научный центр РАМН
Kira Sergeevna Momotyuk
ГУ Гематологический научный центр РАМН
Email: kira@blood.ru
зав. лабораторией крионоконсервирования костного мозга и биологии стволовых клеток, с. н. с, к. м. н; ГУ Гематологический научный центр РАМН
Elena Alekseevna Mikhaylova
ГУ Гематологический научный центр РАМН
Email: mea@blood.ru
отд. химиотерапии гемобластозов и депрессий кроветворения, в. н. с; ГУ Гематологический научный центр РАМН
Nina Iosifovna Drize
ГУ Гематологический научный центр РАМН
Email: ndrize@yandex.ru
зав. лабораторией физиологии кроветворения; ГУ Гематологический научный центр РАМН
D A Svinareva
T V Petrova
I N Shipunova (Nifontova)
K S Momotyuk
E A Mikhailova
N I Drize
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