Leukemic transformation of donor cells: is it possible?
- Authors: Vinogradova OA1, Savchenko VG1, Domracheva EV1, Parovichnikova EN1, Dyachenko LV1, Alimova GA1, Mendeleeva LP1, Lyubimova LS1, Sokolov AN1, Zhelnova EI1, Pokrovskaya OS1
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Affiliations:
- ГНЦ РАМН
- Issue: Vol 79, No 7 (2004)
- Pages: 28-34
- Section: Editorial
- URL: https://journals.rcsi.science/0040-3660/article/view/29991
- ID: 29991
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Abstract
Material and methods. Standard cytogenetics and fluorescent hybridization in situ (FISH) with a probe to the centrometic sites of X/Y chromosomes were used in examination of 2 patients with acute promyelocyte and acute non-differentiated leukemia after allogenic TBM from donors of the opposite gender. Bone marrow was studied 1, 2, 3, 6, 9, 12, 15, 17, 18 months after the transplantation. Results. One of the patient in leukemia recurrence there were 72% cells with one X chromosome with unknown origin 28% donor cells were with genotype XX. The primary archival cytological sample of the recipients bone marrow 68% cells did not contain Ychromosome. Thus, the clone with Yloss is the recipient's clone and leukemia after transplantation developed from the recipient's cells The other
patient had only 8% dividing cells with her karyotype XX with translocation t(10; 11) while 92% metaphases were donor's ones; the interphase cells ratio was 75% of host cells and 25% donor cells. This confirms leukemia origin from the recipient's cells.
Conclusion. High sensitive quantitative method FISH indicates a true correlation between the host and donor cells and is a method of choice for genotyping leukemic cells in recurrence after transplantation of bone marrow. While standard caryotyping depends on mytotic activity of donor and host cell populations, use of only one cytogenetic test for determination of leukemia origin after ТВМ may provoke diagnostic errors.
Keywords
About the authors
O A Vinogradova
ГНЦ РАМНМосква; ГНЦ РАМН
V G Savchenko
ГНЦ РАМНМосква; ГНЦ РАМН
E V Domracheva
ГНЦ РАМНМосква; ГНЦ РАМН
E N Parovichnikova
ГНЦ РАМНМосква; ГНЦ РАМН
L V Dyachenko
ГНЦ РАМНМосква; ГНЦ РАМН
G A Alimova
ГНЦ РАМНМосква; ГНЦ РАМН
L P Mendeleeva
ГНЦ РАМНМосква; ГНЦ РАМН
L S Lyubimova
ГНЦ РАМНМосква; ГНЦ РАМН
A N Sokolov
ГНЦ РАМНМосква; ГНЦ РАМН
E I Zhelnova
ГНЦ РАМНМосква; ГНЦ РАМН
O S Pokrovskaya
ГНЦ РАМНМосква; ГНЦ РАМН
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