Study of antitumor effects of human placenta hydrolysate on PC-3, OAW-42, BT-474 cell cultures

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Abstract

Aim. To investigate the antitumor effects of human placenta hydrolysate (HPH) peptides on three hormone-dependent human cell lines: prostate adenocarcinoma, breast carcinoma, and ovarian cancer by metabolic analysis of cell cultures.

Materials and methods. The effect of HPH on tumor and control tumor cell lines was evaluated. Study stages: (A) de novo peptide sequencing by collision-induced dissociation mass spectrometry; (B) detection of peptides with anti-tumor properties; (C) expert analysis of the obtained lists of peptides.

Results. Dose-dependent cytotoxic effects of HPH on three tumor cell lines are shown: PC-3 (human prostate adenocarcinomas), OAW-42 (human ovarian cancer), BT-474 (human breast carcinomas), and IC50 constants (1.3–2.8 mg/ml) were obtained. The analysis of the HPH peptide fraction showed more than 70 peptides with antitumor properties in the composition of this HPH, including kinase inhibitors: mitogen-activated protein kinases, kappa-bi nuclear factor inhibitor kinase, AKT serine/threonine kinase 1, protein kinase C zeta, interleukin-1 receptor-associated kinase 4 and cyclin-dependent kinase 1.

Conclusion. The results of the study indicate not only the oncological safety of the HPH used in therapy but also the mild antitumor effects of this HPH at high concentrations.

About the authors

Olga A. Gromova

Federal Research Center "Computer Science and Control" of the Russian Academy of Sciences

Author for correspondence.
Email: unesco.gromova@gmail.com
ORCID iD: 0000-0002-7663-710X

д-р мед. наук, проф., вед. науч. сотр. ФИЦ ИУ РАН

Russian Federation, Moscow

Marina V. Filimonova

National Medical Research Radiological Centre; Tsyb Medical Radiological Research Center – branch of the National Medical Research Radiological Centre

Email: unesco.gromova@gmail.com
ORCID iD: 0000-0002-9690-4746

д-р мед. наук, д-р биол. наук, проф. ФГБУ «НМИЦ радиологии», зав. лаб. радиационной фармакологии МРНЦ им. А.Ф. Цыба – филиала ФГБУ «НМИЦ радиологии»

Russian Federation, Moscow; Obninsk

Ivan Yu. Torshin

Federal Research Center "Computer Science and Control" of the Russian Academy of Sciences

Email: unesco.gromova@gmail.com
ORCID iD: 0000-0002-2659-7998

канд. физ.-мат. наук, канд. хим. наук, вед. науч. сотр. ФИЦ ИУ РАН

Russian Federation, Moscow

Daria Е. Frolova

Federal Research Center "Computer Science and Control" of the Russian Academy of Sciences; Ivanovo State Medical University

Email: unesco.gromova@gmail.com
ORCID iD: 0000-0002-1912-4607

канд. мед. наук, науч. сотр. ФИЦ ИУ РАН, ассистент каф. онкологии, акушерства и гинекологии ФГБОУ ВО ИГМУ

Russian Federation, Moscow; Ivanovo

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2. Fig. 1. Survival of tumor cells and immortalized fibroblasts vs concentration of human placenta hydrolysates: a – overall plot of cell line survival; b – overall plot of cell line survival (no dispersion).

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