Optimizing heterosurface adsorbent synthesis for liquid chromatography
- Autores: Bogoslovskii S.1, Serdan A.2
-
Afiliações:
- Bauman State Technical University
- Department of Chemistry
- Edição: Volume 90, Nº 3 (2016)
- Páginas: 671-674
- Seção: Physical Chemistry of Separation Processes: Chromatography
- URL: https://journals.rcsi.science/0036-0244/article/view/167953
- DOI: https://doi.org/10.1134/S0036024416030067
- ID: 167953
Citar
Resumo
The structural and geometric parameters of a silica matrix (SM) for the synthesis of heterosurface adsorbents (HAs) are optimized. Modification is performed by shielding the external surfaces of alkyl-modified silica (AS) using human serum albumin and its subsequent crosslinking. The structural and geometric characteristics of the SM, AS, and HA are measured via low-temperature nitrogen adsorption. It is found that the structural characteristics of AS pores with diameters D < 6 nm do not change during HA synthesis, while the volume of pores with diameters of 6 nm < D < 9 nm shrinks slightly due to the adsorption of albumin in the pore orifices. It is established that the volume of pores with diameters D > 9 nm reduces significantly due to adsorption of albumin. It is concluded that silica gel with a maximum pore size distribution close to 5 nm and a minimal proportion of pores with D > 9 nm is optimal for HA synthesis; this allows us to achieve the greatest similarity between the chromatographic retention parameters for HA and AS. The suitability of the synthesized adsorbents for analyzing drugs in biological fluids through direct sample injection is confirmed by chromatography. It was found that the percentage of the protein fraction detected at the outlet of the chromatographic column is 98%.
Sobre autores
S. Bogoslovskii
Bauman State Technical University
Autor responsável pela correspondência
Email: b.su@bmstu.ru
Rússia, Moscow, 105005
A. Serdan
Department of Chemistry
Email: b.su@bmstu.ru
Rússia, Moscow, 119991