The study of a fluorescent biosensor based on polyelectrolyte microcapsules with encapsulated glucose oxidase
- Authors: Kazakova L.I.1, Sirota N.P.1, Sirota T.V.1, Shabarchina L.I.1
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Affiliations:
- Institute of Theoretical and Experimental Biophysics
- Issue: Vol 91, No 9 (2017)
- Pages: 1828-1832
- Section: Biophysical Chemistry
- URL: https://journals.rcsi.science/0036-0244/article/view/169711
- DOI: https://doi.org/10.1134/S0036024417090151
- ID: 169711
Cite item
Abstract
A fluorescent biosensor is synthesized and described. The biosensor consists of polyelectrolyte microcapsules with glucose oxidase (GOx) entrapped in the cavities and an oxygen-sensitive fluorescent indicator Ru(dpp) immobilized in shells, where Ru(dpp) is tris(4,7-diphenyl-1,10-phenanthroline)ruthenium(II) dichloride. The theoretical activity of the encapsulated GOx and the effect storage time and medium composition have on the stability of sensor microcapsules are determined from polarographic measurements. No change in the activity of the encapsulated enzyme and or its loss to the storage medium are detected over the test period. The dispersion medium (water or a phosphate buffer) are shown to have no effect on the activity of microcapsules with immobilized GOx. The described optical sensor could be used as an alternative to electrochemical sensors for in vitro determination of glucose in the clinically important range of concentrations (up to 10 mmol/L).
About the authors
L. I. Kazakova
Institute of Theoretical and Experimental Biophysics
Author for correspondence.
Email: likazakova@rambler.ru
Russian Federation, Pushchino, Moscow oblast, 142290
N. P. Sirota
Institute of Theoretical and Experimental Biophysics
Email: likazakova@rambler.ru
Russian Federation, Pushchino, Moscow oblast, 142290
T. V. Sirota
Institute of Theoretical and Experimental Biophysics
Email: likazakova@rambler.ru
Russian Federation, Pushchino, Moscow oblast, 142290
L. I. Shabarchina
Institute of Theoretical and Experimental Biophysics
Email: likazakova@rambler.ru
Russian Federation, Pushchino, Moscow oblast, 142290