Spectroscopic study of binding of chlorogenic acid with the surface of ZnO nanoparticles
- Authors: Belay A.1, Kim H.K.2, Hwang Y.2
-
Affiliations:
- School of Applied Natural Sciences, Applied Physics Program
- Department of Nanomaterial Engineering and BK 21 Plus Nanoconvergence Technology Division
- Issue: Vol 91, No 9 (2017)
- Pages: 1781-1790
- Section: Physical Chemistry of Nanoclusters and Nanomaterials
- URL: https://journals.rcsi.science/0036-0244/article/view/169704
- DOI: https://doi.org/10.1134/S0036024417090023
- ID: 169704
Cite item
Abstract
Understanding the interaction properties of biological materials with ZnO NPs is fundamental interest in the field of biotechnological applications as well as in the formation of optoelectronic devices. In this research, the binding of ZnO NPs and chlorogenic acid (CGA) were investigated using fluorescence quenching, UV–Vis absorption spectroscopy, Fourier transform infrared (FTIR), Raman spectroscopy, scanning electron microscopy (TEM), and dynamic light scattering (DLS) techniques. The study results indicated the fluorescence quenching between ZnO NPs and CGA rationalized in terms of static quenching mechanism or the formation of nonfluorescent CGA–ZnO. From fluorescence quenching spectral analysis the binding constant (Ka), number of binding sites (n), and thermodynamic properties, were determined. The quenching constants (Ksv) and binding constant (Ka), decrease with increasing the temperature and their binding sites n are 2. The thermodynamic parameters determined using Van’t Hoff equation indicated binding occurs spontaneously involving the hydrogen bond and van der Walls forces played the major role in the reaction of ZnO NPs with CGA. The Raman, SEM, DLS, and Zeta potential measurements were also indicated the differences in the structure, morphology and sizes of CGA, ZnO NPs, and their corresponding CGA–ZnO due to adsorption of CGA on the surface of ZnO NPs
Keywords
About the authors
Abebe Belay
School of Applied Natural Sciences, Applied Physics Program
Author for correspondence.
Email: abebebelay96@gmail.com
Ethiopia, Adama
Hyung Kook Kim
Department of Nanomaterial Engineering and BK 21 Plus Nanoconvergence Technology Division
Email: abebebelay96@gmail.com
Korea, Republic of, Miryang, 627-706
Yoon-Hwae Hwang
Department of Nanomaterial Engineering and BK 21 Plus Nanoconvergence Technology Division
Email: abebebelay96@gmail.com
Korea, Republic of, Miryang, 627-706