Problems in the size exclusion chromatography of poly(N-isopropylacrylamide) on styragel columns
- Authors: Estrin Y.I.1, Perepelitsina E.O.1, Grishchuk A.A.1
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Affiliations:
- Institute of Problems of Chemical Physics
- Issue: Vol 90, No 7 (2016)
- Pages: 1474-1478
- Section: Physical Chemistry of Surface Phenomena. Chromatography
- URL: https://journals.rcsi.science/0036-0244/article/view/168509
- DOI: https://doi.org/10.1134/S0036024416070086
- ID: 168509
Cite item
Abstract
The molecular weights of poly(N-isopropylacrylamide) (PNIPA), calculated according to polystyrene calibration standards upon the elution of THF on styragel columns, appear to be much lower than their actual values determined using independent approaches. This is likely due to interactions between the nitrogen-containing units of PNIPA polymer chains and the sorbent, so the polymer is eluted in the mode intermediate between exclusion and critical. An effective exclusion mode during the elution of PNIPA on a styragel column can be achieved by using an eluent more polar than tetrahydrofuran (particularly, 1-methylpyrrolidone).
About the authors
Ya. I. Estrin
Institute of Problems of Chemical Physics
Author for correspondence.
Email: estriny@icp.ac.ru
Russian Federation, Chernogolovka, Moscow oblast, 142432
E. O. Perepelitsina
Institute of Problems of Chemical Physics
Email: estriny@icp.ac.ru
Russian Federation, Chernogolovka, Moscow oblast, 142432
A. A. Grishchuk
Institute of Problems of Chemical Physics
Email: estriny@icp.ac.ru
Russian Federation, Chernogolovka, Moscow oblast, 142432