Email this article A Method for Fixation of Fish Larvae for Morphological and Genetic Studies
- Authors: Gordeeva N.V.1,2, Kobyliansky S.G.2, Evseenko S.A.2
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Affiliations:
- Vavilov Institute of General Genetics, Russian Academy of Sciences
- Shirshov Institute of Oceanology, Russian Academy of Sciences
- Issue: Vol 59, No 5 (2019)
- Pages: 818-822
- Section: Short Communications
- URL: https://journals.rcsi.science/0032-9452/article/view/168401
- DOI: https://doi.org/10.1134/S0032945219050035
- ID: 168401
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Abstract
Three aqueous buffer solutions that make it possible to stabilize DNA and also preserve the initial body shape and morphological structures are tested for fixation and preservation of deep-sea fishes’ larvae. Based on the quality assessment of DNA and appearance of the larvae 6 months after fixation, the buffer solution containing dimethyl sulfoxide is recommended for use. Unlike ethanol or formalin, this fixative is nontoxic, nonflammable, does not require storage of samples in the refrigerator, and is also compatible with standard DNA extraction methods or commercial kit protocols.
About the authors
N. V. Gordeeva
Vavilov Institute of General Genetics, Russian Academy of Sciences; Shirshov Institute of Oceanology, Russian Academy of Sciences
Author for correspondence.
Email: ribka04@mail.ru
Russian Federation, Moscow, 119991; Moscow, 117997
S. G. Kobyliansky
Shirshov Institute of Oceanology, Russian Academy of Sciences
Email: ribka04@mail.ru
Russian Federation, Moscow, 117997
S. A. Evseenko
Shirshov Institute of Oceanology, Russian Academy of Sciences
Email: ribka04@mail.ru
Russian Federation, Moscow, 117997
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