Optimization of the Method for Determining the Motility Characteristics of Fish spermatozoa Using ImageJ Software and Excel Macros


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Abstract

A method for computer-based determination of the motility characteristics of fish spermatozoa using ImageJ software and a number of original Excel macros that help optimizing the processing of the data obtained using the wrMTrck_Batch plugin are described. The described method has been tested on sperm of the Black Sea turbot Scophthalmus maeoticus.

About the authors

Iu. S. Baiandina

Kovalevsky Institute of Marine Biological Research, Russian Academy of Sciences (IMBR RAS)

Author for correspondence.
Email: sepulturka@mail.ru
Russian Federation, Sevastopol, 299011

A. N. Khanaychenko

Kovalevsky Institute of Marine Biological Research, Russian Academy of Sciences (IMBR RAS)

Email: sepulturka@mail.ru
Russian Federation, Sevastopol, 299011

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