Evaluation of the Chitinolytic and Antibiotic Activity of the  Streptomyces avidinii  INA 01467 and  Micromonospora aurantiaca  INA 01468

G. A. Teregulova; Терегулова Г. А.; O. N. Sineva; Синёва О. Н.; N. N. Markelova; Маркелова Н. Н.; V. S. Sadikova; Садыкова В. С.; G. V. Uvarov; Уваров Г. В.; M. A. Kovalenko; Коваленко М. А.; N. A. Manucharova; Манучарова Н. А.

doi:10.31857/S0032180X22601207

Evaluation of the Chitinolytic and Antibiotic Activity of the Streptomyces avidinii INA 01467 and Micromonospora aurantiaca INA 01468

Authors: Teregulova G.A.¹, Sineva O.N.², Markelova N.N.², Sadikova V.S.², Uvarov G.V.¹, Kovalenko M.A.¹, Manucharova N.A.¹
Affiliations:
1. Lomonosov Moscow State University
2. Research Institute for the Discovery of New Antibiotics named after G.F. Gauze
Issue: No 5 (2023)
Pages: 594-602
Section: ЭКОЛОГИЯ ПОЧВЕННЫХ МИКРООРГАНИЗМОВ
URL: https://journals.rcsi.science/0032-180X/article/view/138073
DOI: https://doi.org/10.31857/S0032180X22601207
EDN: https://elibrary.ru/IEEWMZ
ID: 138073

Cite item

Full Text

Open Access
Restricted Access

Access granted
Restricted Access

Subscription Access

Abstract
About the authors
References
Supplementary files
Statistics

Abstract

The evaluation of antibiotic and chitinolytic activity in actinomycetes isolated from sod-podzolic soil (Albic Retisol (Loamic)) was carried out Moscow region (56°07′15″ N, 37°30′54″ E). Based on cultural and morphological features and analysis of the 16S rRNA gene, the isolated strains were assigned to Streptomyces avidinii INA 01467 and Micromonospora aurantiaca INA 01468. Evaluation of the antibiotic activity of isolated actinomycete cultures showed that the strains exhibit both antibacterial activity against gram-positive bacteria and antifungal activity against collection strains of fungi Sac. cerevisiae INA 01042, C. albicans ATCC 14053, F. oxysporum VKPM F-148. The ability to synthesize antifungal compounds increased when growing on a medium with chitin in the strain Streptomyces avidinii INA 01467, including against the phytopathogenic strain Fusarium oxysporum VKPM F-148. Using the Real-Time PCR (Real-Time-PCR) method, the presence of functional genes of shitA, responsible for the synthesis of group A chitinase enzymes, was established in the studied bacterial strains of Streptomyces and Micromonospora. The largest amount of the gene is found when growing on chitin in the strain Streptomyces avidinii INA 01467 and reaches about 15 × 10³ copies per ml. The results showed the presence of chitinase and antibiotic activity in the studied strains, including against phytopathogenic fungi, which allows them to be used in plant protection technologies and soil bioremediation.

Keywords

multifunctional actinomycetes, molecular biological methods, chitinolytics, functional chitA gene

References

Варламов В.П., Ильина А.В., Шагдарова Б.Ц., Луньков А.П., Мысякина И.С. Хитин/хитозан и его производные // Успехи биологической химии. 2020. Т. 60. С. 317–368. https://doi.org/10.1134/S0006297920140084
Кожевин П.А. Микробные популяции в природе. М.: Изд-во Моск. ун-та, 1989. 175 с.
Манучарова Н.А., Власенко А.Н., Менько Е.В., Звягинцев Д.Г. Специфика хитинолитического микробного комплекса в почвах, инкубируемых при различных температурах // Микробиология. 2011. Т. 80. № 2. С. 219–229. https://doi.org/10.1134/S002626171102010X
Поздняков Л.А., Степанов А.Л., Гасанов М.Э., Семенов М.В., Якименко О.С., Суада А.К., Рай А.Н., Щеголькова Н.М. Влияние лигногумата на биологическую активность почвы о. Бали, Индонезия // Почвоведение. 2020. № 5. С. 601–609. https://doi.org/10.31857/S0032180X20050111
Aly M.M., Sediq A.N., Baghdadi A.M., Amasha R.H. Chitin and chitinases, production, characterization and applications // IOSR J. Pharm. Biol. Sci. 2019. V. 14. P. 36043. https://doi.org/10.9790/3008-1402013643
Bai Y., Eijsink V.G., Kielak A.M., van Veen J.A., de Boer W. Genomic comparison of chitinolytic enzyme systems from terrestrial and aquatic bacteria // Environ. Microbiol. 2016. V. 18. P. 38–49. https://doi.org/10.1111/1462-2920.12545
Bergey’s Manual of Determinative Bacteriology / Eds. J.A. Holt et al. Baltimore etc: Williams and Wilkins, 2012. V. 5. The Actinobacteria. https://doi.org/10.1002/9781118960608
Bhattacharya D., Nagpure A., Gupta R.K. Bacterial chitinases: properties and potential // Crit. Rev. Biotechnol. 2007. V. 27. P. 21028. https://doi.org/10.1080/07388550601168223
Brzezinska M.S., Jankiewicz U., Burkowska A., Walczak M. Chitinolytic microorganisms and their possible application in environmental protection // Curr. Microbiol. 2014. V. 68. P. 71–81. https://doi.org/10.1007/s00284-013-0440-4
Dahiya N., Tewari R., Hoondal G.S. Biotechnological aspects of chitinolytic enzymes: a review // Appl. Microbiol. Biotechnol. 2006. V. 71. P. 7730782. https://doi.org/10.1007/s00253-005-0183-7
Golovchenko A.V., Gracheva T.A., Lypcan V.A., Dobrovol’skaya T.G., Manucharova N.A. Actinomycete Complex es in Eutrophic Peatlands // Eurasian Soil Science. 2022. T. 55. № 8. P. 1064–1073. https://doi.org/10.1134/S1064229322080026
Gschwendtner S., Reichmann M., Müller M., Radl V., Munch J.C., Schloter M. Abundance of bacterial genes encoding for proteases and chitinases in the rhizosphere of three different potato cultivars // Biol. Fertil. Soils. 2010. V. 46. P. 6490652. https://doi.org/10.1007/s00374-010-0460-1
Han Y., Yang B., Zhang F., Miao X., Li Z. Characterization of antifungal chitinase from marine Streptomyces sp. DA11 associated with South China sea sponge Craniella Australiensis // Mar. Biotechnol. 2009. V. 11. P. 132–140. https://doi.org/10.1007/s10126-008-9126-5
Hobel C.F.V., Hreggvidsson G.O., Marteinsson V.T., Bahrani Mougeot F., Einarsson J.M., Kristjansson J.K. Cloning, expression, and characterization of a highly thermostable family 18 chitinase from Rhodothermus marinus // Extremophiles. 2005. V 9. № 1. P. 53064. https://doi.org/10.1007/s00792-004-0422-3
Kashyap D.R., Vohra P.K., Chopra S., Tewari R. Application of pectinases in the commercial sector: a review// Bioresour. Technol. 2001. V. 77. P. 2150237. https://doi.org/10.1016/S0960-8524(00)00118-8
Kumar M., Amandeep Brar A., Yadav M., Chawade A., Vivekanand V., Nidhi Pareek N. Chitinases-potential candidates for enhanced plant resistance towards fungal pathogens // Agricult. 2018. V. 8. № 88. https://doi.org/10.3390/agriculture8070088
Lacombe-Harvey M.E., Brzezinski R., Beaulieu C. Chitinolytic functions in actinobacteria: ecology, enzymes, and evolution // Appl. Microbiol. Biotechnol. 2018. V. 102. P. 7219–7230. https://doi.org/10.1007/s00253-018-9149-4
Manucharova N.A. The microbial destruction of chitin, pectin, and cellulose in soils // Eurasian Soil Science. 2009. V. 42. № 13. P. 1526–1532. https://doi.org/10.1134/S1064229309130146
Nawani N.N., Kapadnis B.D., Das A.D., Rao A.S., Mahajan S.K. Purification and characterization of a thermophilic and acidophilic chitinase from Microbispora sp. V2 // J. Appl. Microbiol. 2002. V. 93. P. 965–975. https://doi.org/10.1046/j.1365-2672.2002.01766.x
Wang Q., Duan B., Yang R., Zhao Y., Zhang L. Screening and identification of chitinolytic actinomycetes and study on the inhibitory activity against Turfgrass Root rot disease fungi // J. Biosci. Medic. 2015. V. 3. P. 56065. https://doi.org/10.4236/jbm.2015.33009
Williamson N., Brian, P., Wellington E.M. Molecular detection of bacterial and streptomycete chitinase in the environment // Antonie Van Leeuwenhoek. 2000. V. 78. P. 315–321. https://doi.org/10.1023/A:1010225909148
Xiao X., Yin X., Lin J., Sun L., You Z., Wang P., Wang F. Chitinase Genes in Lake Sediments of Ardley Island, Antarctica // Appl. Environ. Microbiol. 2005. V. 71. P. 790407909. https://doi.org/10.1128/AEM.71.12.7904-7909.2005