NMR Relaxometry at Quantification of the Captured Magnetic Nanoparticles by Cells


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Abstract

The possibility of using the transverse relaxation time T2 of protons in aqueous media for quantitative measurement of the capture of magnetic nanoparticles by cells has been studied and demonstrated. The measurement of T2 was performed on a portable original NMR relaxometer with a measuring cell for a standard well of a biological plate. The novelty of the approach is that quantitative measurements of the capture kinetics were carried out using measurements of the proton relaxation time of the nutrient medium, which is determined by the remaining number of magnetic particles (not captured by the cells) in the medium. To study the kinetics of capture, two types of magnetic nanoparticles were synthesized: magnetite particles Fe3O4 and composite particles Fe@C with an iron-carbon shell structure. The surface of the particles was functionalized with amine-and carboxyl groups. The capture of aminated particles of Fe@C cells is established by microscopy and NMR-relaxometry by measuring the time T2. It is shown that the proposed method makes it possible to register very small concentrations of trapped magnetic nanoparticles equal to tens of pg/cell.

About the authors

I. V. Byzov

Mikheev Institute of Metal Physics, Ural Branch, Russian Academy of Sciences

Author for correspondence.
Email: ivbyzov@gmail.com
Russian Federation, Ekaterinburg, 620108

V. P. Krasnov

Postovsky Institute of Organic Synthesis, Ural Branch, Russian Academy of Sciences

Email: ivbyzov@gmail.com
Russian Federation, Ekaterinburg, 620137

A. M. Demin

Postovsky Institute of Organic Synthesis, Ural Branch, Russian Academy of Sciences

Email: ivbyzov@gmail.com
Russian Federation, Ekaterinburg, 620137

M. V. Ulitko

Ural Federal University Named after the First President of Russia B.N. Yeltsin

Email: ivbyzov@gmail.com
Russian Federation, Ekaterinburg, 620002

S. V. Zhakov

Mikheev Institute of Metal Physics, Ural Branch, Russian Academy of Sciences

Email: ivbyzov@gmail.com
Russian Federation, Ekaterinburg, 620108

M. B. Rayev

Institute of Ecology and Genetic of Micriorganisms, Ural Branch, Russian Academy of Sciences

Email: ivbyzov@gmail.com
Russian Federation, Perm, 614081

A. A. Mysik

Mikheev Institute of Metal Physics, Ural Branch, Russian Academy of Sciences

Email: ivbyzov@gmail.com
Russian Federation, Ekaterinburg, 620108

A. E. Yermakov

Mikheev Institute of Metal Physics, Ural Branch, Russian Academy of Sciences; Ural Federal University Named after the First President of Russia B.N. Yeltsin; Institute of Ecology and Genetic of Micriorganisms, Ural Branch, Russian Academy of Sciences

Email: ivbyzov@gmail.com
Russian Federation, Ekaterinburg, 620108; Ekaterinburg, 620002; Perm, 614081

M. A. Uimin

Mikheev Institute of Metal Physics, Ural Branch, Russian Academy of Sciences; Ural Federal University Named after the First President of Russia B.N. Yeltsin; Institute of Ecology and Genetic of Micriorganisms, Ural Branch, Russian Academy of Sciences

Email: ivbyzov@gmail.com
Russian Federation, Ekaterinburg, 620108; Ekaterinburg, 620002; Perm, 614081

A. S. Minin

Mikheev Institute of Metal Physics, Ural Branch, Russian Academy of Sciences; Ural Federal University Named after the First President of Russia B.N. Yeltsin; Institute of Ecology and Genetic of Micriorganisms, Ural Branch, Russian Academy of Sciences

Email: ivbyzov@gmail.com
Russian Federation, Ekaterinburg, 620108; Ekaterinburg, 620002; Perm, 614081

I. V. Zubarev

Ural Federal University Named after the First President of Russia B.N. Yeltsin; Moscow Institute of Physics and Technology

Email: ivbyzov@gmail.com
Russian Federation, Ekaterinburg, 620002; Moscow, 115184

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