Spectral Analysis of Organic Components of Demineralized Bone Biografts

Abstract

The results of an advanced spectral analysis of human donor bone grafts obtained using Raman spectroscopy are presented. Mathematical methods were applied to improve the resolution of spectral profiles, and chemometric principal component analysis was used to evaluate the organic components of the grafts. The results were compared to the proteomic data obtained using mass spectrometry. The main differences between cadaver and intraoperative grafts were observed in the Raman spectral lines at 1450 and 1735 cm^–1 (lipids and fatty acids), 917 and 937 cm^–1 (glycogen), and 814 and 894 cm^–1 (DNA), as well as 1238 and 1560 cm^–1 (amide II and amide III). It is shown that Raman spectroscopy can be employed to evaluate relative concentrations of components of DNA, RNA, collagen, protein, extracellular matrix, and bone tissue.