Photoinduced Enhancement of Evans Blue Dye Fluorescence in Water Solution of Albumin

Resumo

The effect of photoinduced enhancement of Evans blue (EB) dye fluorescence in blood plasma and albumin solution has been considered. Manifestations of the dye fluorescence enhancement in the albumin solution and rat blood plasma have been compared. Saturation of photoinduced fluorescence, the effect of enhanced fluorescence delay, and divergence in fluorescence spectra of the solutions have been found for the first time. Based on the obtained results, a hypothesis about the presence of nonfluorescent complexes with the EB participation in blood plasma is proposed.