Complexes of the ATP-dependent Lon protease and DNA aptamers with G-quadruplexes as a model for developing a nanosensor biomagnetic immunoassay system

V. A. Spiridonova; V. A. Sizov; E. O. Kuzmenko; A. V. Melnichuk; E. A. Oleinichenko; A. M. Kudzhaev; T. V. Rotanova; O. V. Snigirev

doi:10.3103/S0027134917040130

Complexes of the ATP-dependent Lon protease and DNA aptamers with G-quadruplexes as a model for developing a nanosensor biomagnetic immunoassay system

Autores: Spiridonova V.A.¹, Sizov V.A.², Kuzmenko E.O.², Melnichuk A.V.¹, Oleinichenko E.A.¹, Kudzhaev A.M.³, Rotanova T.V.³, Snigirev O.V.⁴
Afiliações:
1. Belozersky Institute of Physicochemical Biology
2. Faculty of Chemistry
3. Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry
4. Faculty of Physics
Edição: Volume 72, Nº 4 (2017)
Páginas: 376-382
Seção: Biophysics and Medical Physics
URL: https://journals.rcsi.science/0027-1349/article/view/164807
DOI: https://doi.org/10.3103/S0027134917040130
ID: 164807

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The binding to Lon protease through biotinylated aptamers whose structures contain G-quadruplex fragments with magnetic nanoparticles (MNPs) functionalized by streptavidin was investigated. The conditions of binding of target aptamers to MNPs are met. The resulting complexes are proposed for detection of Lon protease in different biological sources and for constructing a novel biomagnetic nanosensor immunoassay system.