The preparation and study of the properties of macroporous monolith-based continuous flow bioreactors

Abstract

The surfaces of macroporous monolithic disks were modified with hydrolytic enzymes, viz., α-chymotrypsin and ribonuclease A. The effect of the enzyme-immobilization approach and the amount of the aldehyde groups in the polymer spacer, as well as the flow rate of the substrate solution on the efficiency of the heterogeneous biocatalysis was explored using the reactions of hydrolysis of both low and macromolecular substrates as examples.