Отправить статью по E-mail The Role of Charged Residues in the Structural Adaptation of Short-Chain Alcohol Dehydrogenase (SDR) from Thermophilic Organisms to High Temperatures
- Авторы: Popinako A.V.1, Antonov M.Y.2, Bezsudnova E.Y.1, Popov V.O.1,3
-
Учреждения:
- Federal Research Centre Fundamentals of Biotechnology
- Ammosov North-Eastern Federal University
- National Research Centre Kurchatov Institute
- Выпуск: Том 73, № 5 (2018)
- Страницы: 231-236
- Раздел: Article
- URL: https://journals.rcsi.science/0027-1314/article/view/163778
- DOI: https://doi.org/10.3103/S0027131418050085
- ID: 163778
Цитировать
Открытый доступ
Доступ предоставлен
Только для подписчиков
Аннотация
Understanding the adaptation mechanisms of proteins from extremophiles has paved the way for the development of new biocatalysts resistant to an extreme proton deficit. In the present work, we study the structural adaptation of NADP-dependent short-chain alcohol dehydrogenase/reductase (SDR) to high temperatures. We present the analysis of the amino acid composition of the SDR sequences, the comparative analysis of the structural elements in the SDR from mesophiles and thermophiles, and the results of the molecular dynamics of the superthermostable short-chain alcohol dehydrogenase from the hyperthermophilic archaeon Thermococcus sibiricus (TsAdh319) and its homologues.
Ключевые слова
Об авторах
A. Popinako
Federal Research Centre Fundamentals of Biotechnology
Автор, ответственный за переписку.
Email: popinakoav@gmail.com
Россия, Moscow, 119071
M. Antonov
Ammosov North-Eastern Federal University
Email: popinakoav@gmail.com
Россия, Republic of Sakha (Yakutia), 677007
E. Bezsudnova
Federal Research Centre Fundamentals of Biotechnology
Email: popinakoav@gmail.com
Россия, Moscow, 119071
V. Popov
Federal Research Centre Fundamentals of Biotechnology; National Research Centre Kurchatov Institute
Email: popinakoav@gmail.com
Россия, Moscow, 119071; Moscow, 123098
Дополнительные файлы
