Separation of water-soluble vitamins on silica modified with L-cysteine–stabilized gold nanoparticles using HPLC

Abstract

Silica functionalized with 3-aminopropyltriethoxysilane and L-cysteine–stabilized gold nanoparticles has been obtained (SiO₂–NH₂–Au–L-cysteine). The influence of pH and the content of acetonitrile in the mobile phase on retention and separation selectivity of eight vitamins has been studied. The chromatographic conditions that enable the separation of C, B3, B12, B5 and B1, B2, B6, B10 mixtures of vitamins have been proposed. It has been found that it is possible to separate a mixture of vitamins C, B3, B12, and B5 in 8 min, and a mixture of vitamins B1, B2, B6, B10 in 12 min on the synthesized sorbent SiO₂–NH₂–Au–L-cysteine in isocratic elution mode.