Anti-Restriction Activity of ArdB Protein against EcoAI Endonuclease

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Аннотация

ArdB proteins are known to inhibit the activity of type I restriction–modification (RM-I) system, in particular EcoKI (family IA). The mechanism of ArdB’s activity still remains unknown; the spectrum of targets inhibited by them has been poorly studied. In this work, it was shown that the presence of the ardB gene from R64 plasmid could suppress the activity of EcoAI endonuclease (IB family) in Escherichia coli TG1 cells. The absence of specificity of ArdB to a certain RM-I system (it inhibits both the IA- and IB-family), it can be a-ssumed that the mechanism of the anti-restriction activity of this protein does not depend on both the s-equence DNA at the recognition site and the structure of the restrictase of the RM-I systems.

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Авторлар туралы

A. Kudryavtseva

Moscow Institute of Physics and Technology

Хат алмасуға жауапты Автор.
Email: kudryavtseva@phystech.edu
Russia, 141707, Moscow Region, Dolgoprudny

V. Alekhin

“Biophystech” LLC

Email: kudryavtseva@phystech.edu
Russia, 141701, Moscow Region, Dolgoprudny

M. Lebedeva

“Biophystech” LLC

Email: kudryavtseva@phystech.edu
Russia, 141701, Moscow Region, Dolgoprudny

E. Csefalvay

Laboratory of Structural Biology and Bioinformatics, Institute of Microbiology, Academy of Sciences of the Czech Republic

Email: kudryavtseva@phystech.edu
Czech Republic, 37333 , Nove Hrady, Zamek 136

M. Weiserova

Institute of Microbiology, Academy of Sciences of the Czech Republic

Email: kudryavtseva@phystech.edu
Czech Republic, 14220, Praha 4,, Vídeňská 1083

I. Manukhov

Moscow Institute of Physics and Technology; Moscow State University of Food Production

Email: kudryavtseva@phystech.edu
Russia, 141707, Moscow Region, Dolgoprudny; Russia, 125080, Moscow

Әдебиет тізімі

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© А.А. Кудрявцева, В.А. Алехин, М.Д. Лебедева, E. Csefalvay, M. Weiserova, И.В. Манухов, 2023

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