电邮这篇文章 In Vitro Reconstitution of the S. aureus 50S Ribosomal Subunit and GTP-Binding Factor YSXC Complex for Structural Studies
- 作者: Biktimirov A.D.1, Yusupov M.M.1,2,3, Usachev K.S.1
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隶属关系:
- Kazan Federal University
- National Research Center “Kurchatov Institute”
- Institute of Genetics, Molecular and Cellular Biology, CNRS UMR7104, INSERM U964
- 期: 卷 59, 编号 4 (2025)
- 页面: 607-615
- 栏目: МОЛЕКУЛЯРНАЯ БИОЛОГИЯ КЛЕТКИ
- URL: https://journals.rcsi.science/0026-8984/article/view/320600
- DOI: https://doi.org/10.31857/S0026898425040078
- ID: 320600
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详细
The proper assembly and maturation of ribosomal subunits are critical processes that ensure the functional activity, translation efficiency, and fidelity of the ribosome. One of the protein factors involved in the processes of ribosome large subunit maturation is the GTP-binding protein YsxC, which is found in many bacteria. In the absence of YsxC, immature ribosomal intermediates, designated 45S subunits, are observed to accumulate within the cell. These are unable to associate with the small subunit of the ribosome and thus fail to form ribosomes capable of carrying out the necessary functions. The deletion of the ysxC gene is lethal to S. aureus. The mechanism of YsxC interaction with the Staphylococcus aureus ribosome remains to be elucidated. In this study, we devised a protocol for the isolation, purification, and assembly of the YsxC protein complex with the 50S subunit of the Staphylococcus aureus ribosome, which enabled us to obtain a sample suitable for registration of transmission cryo-electron microscopy data.
作者简介
A. Biktimirov
Kazan Federal UniversityKazan, 420008 Russia
M. Yusupov
Kazan Federal University; National Research Center “Kurchatov Institute”; Institute of Genetics, Molecular and Cellular Biology, CNRS UMR7104, INSERM U964Kazan, 420008 Russia; Moscow, 123182 Russia; Universit’e de Strasbourg, Illkirch, F‑67400 France
K. Usachev
Kazan Federal University
Email: konstantin.usachev@kpfu.ru
Kazan, 420008 Russia
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