Search for Functionally Significant Motifs and Amino Acid Residues of Actin


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Аннотация

The scientific interest to the structural and functional properties of actin is determined by its abundance in cells. Being an important component of the cytoskeleton, actin is involved in many protein-protein interactions. Using crystal structures and molecular models, we have mapped the amino acid residues that are involved in these interactions and form the ATP-binding site of the actin monomer. Moreover, using mass spectrometry and high-performance liquid chromatography methods, we have discovered the regions of the amino acid sequence of actin that form the core of the actin fibril. According to the bioinformatic analysis, these regions are amyloidogenic and are located in the C-terminal region and in the hinge between the first and third subdomains. The data obtained are applicable to chordate actin, because multiple alignment revealed highly conserved amino acid sequences. In turn, the comparison of the chordate actin with the bacterial homologs showed the presence of numerous amino acid substitutions and insertions.

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Авторлар туралы

T. Tikhomirova

Institute of Protein Research; Institute for Biological Instrumentation

Email: ogalzit@vega.protres.ru
Ресей, Pushchino, Moscow oblast, 142290; Pushchino, Moscow oblast, 142290

R. Ievlev

Institute of Protein Research

Email: ogalzit@vega.protres.ru
Ресей, Pushchino, Moscow oblast, 142290

M. Suvorina

Institute of Protein Research

Email: ogalzit@vega.protres.ru
Ресей, Pushchino, Moscow oblast, 142290

L. Bobyleva

Institute of Theoretical and Experimental Biophysics

Email: ogalzit@vega.protres.ru
Ресей, Pushchino, Moscow oblast, 142290

I. Vikhlyantsev

Institute of Theoretical and Experimental Biophysics; Pushchino State Institute of Natural Science

Email: ogalzit@vega.protres.ru
Ресей, Pushchino, Moscow oblast, 142290; Pushchino, Moscow oblast, 142290

A. Surin

Institute of Protein Research; State Research Center for Applied Microbiology and Biotechnology

Email: ogalzit@vega.protres.ru
Ресей, Pushchino, Moscow oblast, 142290; Obolensk, Moscow oblast, 142279

O. Galzitskaya

Institute of Protein Research

Хат алмасуға жауапты Автор.
Email: ogalzit@vega.protres.ru
Ресей, Pushchino, Moscow oblast, 142290

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© Pleiades Publishing, Inc., 2018