Differences in Titin and Nebulin Gene Expression in Skeletal Muscles of Rats Chronically Alcoholized by Different Methods
- Authors: Gritsyna Y.V.1, Ulanova A.D.1,2, Salmov N.N.1, Bobylev A.G.1,2, Zhalimov V.K.3, Vikhlyantsev I.M.1,2
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Affiliations:
- Institute of Theoretical and Experimental Biophysics, Russian Academy of Sciences
- Pushchino State Institute of Natural Sciences
- Institute of Cell Biophysics, Russian Academy of Sciences
- Issue: Vol 53, No 1 (2019)
- Pages: 54-63
- Section: Molecular Cell Biology
- URL: https://journals.rcsi.science/0026-8933/article/view/163799
- DOI: https://doi.org/10.1134/S0026893319010035
- ID: 163799
Cite item
Abstract
This work studied the changes in the levels of the main proteins of the calpain system (μ-calpain, Са2+-dependent protease, and fragments of its autolysis, inhibitor calpastatin) and μ-calpain substrates (giant proteins of the sarcomere cytoskeleton, titin and nebulin) in skeletal muscle (m. gastrocnemius, m. soleus, m. longissimus dorsi) of rats alcoholized for three months by different methods using agar containing 30% ethanol and nutrient-balanced liquid feed containing 5% ethanol using gel electrophoresis methods under denaturing conditions and immunoblotting. No decrease in the muscle mass/body weight ratio, indicating the development of atrophy, no increase in autolysis of μ-calpain, indicating an increase in the activity of this enzyme, no changes in the content of intact titin (T1), nebulin, μ-calpain and calpastatin, as well as the total calpain activity measured using Calpain Activity Assay Kit were detected in alcoholized rats of both groups. No changes in the total level of titin phosphorylation in the rat muscles of alcoholized groups were detected using Pro-Q Diamond fluorescent dye for phosphate groups of proteins. No statistically significant differences in the content of titin and nebulin mRNA in skeletal muscles of control rats and rats alcoholized using agar were detected. In rats, alcoholized by the method of liquid feed, the levels of titin and nebulin mRNA were increased 1.5–2.5 times possibly due to a higher fat content in such a diet. The presented data may be useful for choosing a chronic alcoholization model for animals.
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About the authors
Yu. V. Gritsyna
Institute of Theoretical and Experimental Biophysics, Russian Academy of Sciences
Author for correspondence.
Email: gri23.86@mail.ru
Russian Federation, Pushchino, Moscow oblast, 142290
A. D. Ulanova
Institute of Theoretical and Experimental Biophysics, Russian Academy of Sciences; Pushchino State Institute of Natural Sciences
Email: gri23.86@mail.ru
Russian Federation, Pushchino, Moscow oblast, 142290; Pushchino, Moscow oblast, 142290
N. N. Salmov
Institute of Theoretical and Experimental Biophysics, Russian Academy of Sciences
Email: gri23.86@mail.ru
Russian Federation, Pushchino, Moscow oblast, 142290
A. G. Bobylev
Institute of Theoretical and Experimental Biophysics, Russian Academy of Sciences; Pushchino State Institute of Natural Sciences
Email: gri23.86@mail.ru
Russian Federation, Pushchino, Moscow oblast, 142290; Pushchino, Moscow oblast, 142290
V. K. Zhalimov
Institute of Cell Biophysics, Russian Academy of Sciences
Email: gri23.86@mail.ru
Russian Federation, Pushchino, Moscow oblast, 142290
I. M. Vikhlyantsev
Institute of Theoretical and Experimental Biophysics, Russian Academy of Sciences; Pushchino State Institute of Natural Sciences
Email: gri23.86@mail.ru
Russian Federation, Pushchino, Moscow oblast, 142290; Pushchino, Moscow oblast, 142290
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