Email this article In vitro Antiviral Activity of Recombinant Antibodies of IgG and IgA Isotypes to Hemagglutinin of the Influenza A Virus
- Authors: Argentova V.V.1, Aliev T.K.2, Zarubaev V.V.3, Klotchenko S.A.3, Shtro A.A.3, Sergeeva M.V.3, Toporova V.A.4, Dolgikh D.A.1,4, Sveshnikov P.G.5, Vasin V.A.3, Kirpichnikov M.P.1,4
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Affiliations:
- Department of Bioengineering, Faculty of Biology
- Department of Chemical Enzymology, Faculty of Chemistry
- Research Institute of Influenza
- Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry
- Russian Research Center for Molecular Diagnostics and Therapy
- Issue: Vol 51, No 6 (2017)
- Pages: 804-812
- Section: Current Trends in the Application of Monoclonal Antibodies Special Issue
- URL: https://journals.rcsi.science/0026-8933/article/view/163293
- DOI: https://doi.org/10.1134/S0026893317060024
- ID: 163293
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Abstract
Seasonal and highly infectious strains of the influenza A and influenza B viruses cause millions of cases of severe complications in elderly people, children, and patients with immune diseases each year. Immunoglobulin A (IgA), which is an active component of humoral immunity, can prevent the spread of the virus in the upper respiratory tract. The preparation and study of the properties of recombinant virus-specific IgA could be an important approach to finding new means of preventing and treating influenza. Based on CHO DG44 cells, we developed stable monoclonal cell lines that produce monomeric and dimeric antibodies FI6-IgA1 and FI6-IgA2m1 to hemagglutinin (HA) of the influenza A virus. When studying the productivity, growth, and stability of the obtained clones, we found that the dimeric form of antibodies of IgA1 isotype is superior to other forms. The dimeric form of IgA antibodies plays a key role in mucosal immunity. Recognizing the prospects of using dimeric IgA as prophylactic and therapeutic mucosal drugs for viral infections, we studied their virus-neutralizing and antiviral activities on MDCK cell culture and compared them with the antibodies of the IgG1 isotype. This study presents the data on antiviral and virus-neutralizing activities of the FI6-IgA1 dimers to seasonal and highly infectious strains of influenza A virus.
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About the authors
V. V. Argentova
Department of Bioengineering, Faculty of Biology
Author for correspondence.
Email: vicarg@rambler.ru
Russian Federation, Moscow, 119234
T. K. Aliev
Department of Chemical Enzymology, Faculty of Chemistry
Email: vicarg@rambler.ru
Russian Federation, Moscow, 119991
V. V. Zarubaev
Research Institute of Influenza
Email: vicarg@rambler.ru
Russian Federation, St. Petersburg, 197376
S. A. Klotchenko
Research Institute of Influenza
Email: vicarg@rambler.ru
Russian Federation, St. Petersburg, 197376
A. A. Shtro
Research Institute of Influenza
Email: vicarg@rambler.ru
Russian Federation, St. Petersburg, 197376
M. V. Sergeeva
Research Institute of Influenza
Email: vicarg@rambler.ru
Russian Federation, St. Petersburg, 197376
V. A. Toporova
Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry
Email: vicarg@rambler.ru
Russian Federation, Moscow, 117997
D. A. Dolgikh
Department of Bioengineering, Faculty of Biology; Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry
Email: vicarg@rambler.ru
Russian Federation, Moscow, 119234; Moscow, 117997
P. G. Sveshnikov
Russian Research Center for Molecular Diagnostics and Therapy
Email: vicarg@rambler.ru
Russian Federation, Moscow, 117149
V. A. Vasin
Research Institute of Influenza
Email: vicarg@rambler.ru
Russian Federation, St. Petersburg, 197376
M. P. Kirpichnikov
Department of Bioengineering, Faculty of Biology; Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry
Email: vicarg@rambler.ru
Russian Federation, Moscow, 119234; Moscow, 117997
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