Email this article Characterization of Pectobacterium carotovorum subsp. carotovorum Bacteriophage PP16 Prospective for Biocontrol of Potato Soft Rot
- Authors: Voronina M.V.1, Bugaeva E.N.1, Vasiliev D.M.1, Kabanova A.P.1,2, Barannik A.P.2, Shneider M.M.2, Kulikov E.E.3, Korzhenkov A.A.4,5, Toschakov S.V.3,4, Ignatov A.N.1, Miroshnikov K.A.1,2
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Affiliations:
- PhytoEngineering Research Center
- Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences
- Winogradsky Institute of Microbiology, Research Center of Biotechnology, Russian Academy of Sciences
- Kant Baltic Federal University
- Kurchatov Institute
- Issue: Vol 88, No 4 (2019)
- Pages: 451-460
- Section: Experimental Articles
- URL: https://journals.rcsi.science/0026-2617/article/view/164055
- DOI: https://doi.org/10.1134/S0026261719040118
- ID: 164055
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Abstract
Phages of the phytopathogenic Pectobacteriaceae species causing black leg and soft rot of potato were investigated. These phages are promising as biocontrol agents to prevent the loss of seed and ware potato tubers. The present work characterizes a new podovirus PP16, infecting a broad range of Pectobacterium carotovorum strains. Based on its genomic composition, phage PP16 was assigned to a separate phylogenetic branch of the genus Phimunavirus, subfamily Autographivirinae. Bacteriophage PP16 efficiently inhibited development of bacterial infection both in vitro and in planta. The field experiment demonstrated a substantial increase of plant germination after the treatment of seed potato with phage PP16.
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About the authors
M. V. Voronina
PhytoEngineering Research Center
Email: kmi@ibch.ru
Russian Federation, Rogachevo, Moscow region, 141880
E. N. Bugaeva
PhytoEngineering Research Center
Email: kmi@ibch.ru
Russian Federation, Rogachevo, Moscow region, 141880
D. M. Vasiliev
PhytoEngineering Research Center
Email: kmi@ibch.ru
Russian Federation, Rogachevo, Moscow region, 141880
A. P. Kabanova
PhytoEngineering Research Center; Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences
Email: kmi@ibch.ru
Russian Federation, Rogachevo, Moscow region, 141880; Moscow, 117997
A. P. Barannik
Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences
Email: kmi@ibch.ru
Russian Federation, Moscow, 117997
M. M. Shneider
Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences
Email: kmi@ibch.ru
Russian Federation, Moscow, 117997
E. E. Kulikov
Winogradsky Institute of Microbiology, Research Center of Biotechnology, Russian Academy of Sciences
Email: kmi@ibch.ru
Russian Federation, Moscow, 119071
A. A. Korzhenkov
Kant Baltic Federal University; Kurchatov Institute
Email: kmi@ibch.ru
Russian Federation, Kaliningrad,, 236041; Moscow, 123098
S. V. Toschakov
Winogradsky Institute of Microbiology, Research Center of Biotechnology, Russian Academy of Sciences; Kant Baltic Federal University
Email: kmi@ibch.ru
Russian Federation, Moscow, 119071; Kaliningrad,, 236041
A. N. Ignatov
PhytoEngineering Research Center
Email: kmi@ibch.ru
Russian Federation, Rogachevo, Moscow region, 141880
K. A. Miroshnikov
PhytoEngineering Research Center; Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences
Author for correspondence.
Email: kmi@ibch.ru
Russian Federation, Rogachevo, Moscow region, 141880; Moscow, 117997
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