Email this article Members of the Uncultured Taxon OP1 (“Acetothermia”) Predominate in the Microbial Community of an Alkaline Hot Spring at East-Tuvinian Upland
- Authors: Korzhenkov A.A.1, Teplyuk A.V.1, Lebedinsky A.V.2, Khvashchevskaya A.A.3, Kopylova Y.G.3, Arakchaa K.D.4, Golyshin P.N.5, Lunev E.A.1, Golyshina O.V.5, Kublanov I.V.2, Toshchakov S.V.2, Gavrilov S.N.2
-
Affiliations:
- Immanuel Kant Baltic Federal University
- Winogradsky Institute of Microbiology, Research Center of Biotechnology, Russian Academy of Sciences
- Tomsk Polytechnic University
- Research Institute of Medico-Social Problems of Tyva Republic
- Bangor University
- Issue: Vol 87, No 6 (2018)
- Pages: 783-795
- Section: Experimental Articles
- URL: https://journals.rcsi.science/0026-2617/article/view/163750
- DOI: https://doi.org/10.1134/S0026261718060115
- ID: 163750
Cite item
Open Access
Access granted
Subscription Access
Abstract
The thermophilic microbial community of a hydrotherm at East-Tuvinian upland is described. High-throughput profiling of the 16S rRNA gene and sequencing of the complete metagenome were used to characterize the phylogenetic and metabolic diversity of the microbial community of the Dikij Arjaan alkaline thermal spring of Ush-Bel’dir mineral water deposit in Tyva, Russia. Members of the uncultured phylum “Acetothermia” (candidate division OP1) predominated in the microbial community of the spring. While this phylum has already been detected in many thermal ecosystems, it never formed the main component of their microbial communities. Apart from “Acetothermia”, members of Nitrospirae and Chloroflexi comprised a significant part of the microbial community. Comparative analysis of our geochemical data on the spring and the previously published data on in silico reconstruction of “Acetothermia” metabolism led us to the suggestion that carbonate and bicarbonate can serve as the major carbon sources for the dominant bacterial group and that “Acetothermia” act as the primary producers in this ecosystem. Analysis of the functional genes also revealed the presence of physiological groups of denitrifyers, iron reducers, carboxydotrophs and diazotrophs. This is the first report on a thermophilic microbial community of a hydrotherm from the southwestern extremity of the Baikal rift zone. Moreover, microbial communities with predominance of uncultured “Acetothermia” have not been reported previously in terrestrial hydrotherms.
About the authors
A. A. Korzhenkov
Immanuel Kant Baltic Federal University
Email: sngavrilov@gmail.com
Russian Federation, Kaliningrad
A. V. Teplyuk
Immanuel Kant Baltic Federal University
Email: sngavrilov@gmail.com
Russian Federation, Kaliningrad
A. V. Lebedinsky
Winogradsky Institute of Microbiology, Research Center of Biotechnology, Russian Academy of Sciences
Email: sngavrilov@gmail.com
Russian Federation, Moscow
A. A. Khvashchevskaya
Tomsk Polytechnic University
Email: sngavrilov@gmail.com
Russian Federation, Tomsk
Yu. G. Kopylova
Tomsk Polytechnic University
Email: sngavrilov@gmail.com
Russian Federation, Tomsk
K. D. Arakchaa
Research Institute of Medico-Social Problems of Tyva Republic
Email: sngavrilov@gmail.com
Russian Federation, Kyzyl
P. N. Golyshin
Bangor University
Email: sngavrilov@gmail.com
United Kingdom, Gwynedd
E. A. Lunev
Immanuel Kant Baltic Federal University
Email: sngavrilov@gmail.com
Russian Federation, Kaliningrad
O. V. Golyshina
Bangor University
Email: sngavrilov@gmail.com
United Kingdom, Gwynedd
I. V. Kublanov
Winogradsky Institute of Microbiology, Research Center of Biotechnology, Russian Academy of Sciences
Email: sngavrilov@gmail.com
Russian Federation, Moscow
S. V. Toshchakov
Winogradsky Institute of Microbiology, Research Center of Biotechnology, Russian Academy of Sciences
Email: sngavrilov@gmail.com
Russian Federation, Moscow
S. N. Gavrilov
Winogradsky Institute of Microbiology, Research Center of Biotechnology, Russian Academy of Sciences
Author for correspondence.
Email: sngavrilov@gmail.com
Russian Federation, Moscow
Supplementary files
