Characterization of Carbohydrate-Containing Components of Azospirillum brasilense Sp245 Biofilms


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Abstract

The structure and functions of the biopolymers of the matrix of Azospirillum brasilense biofilms formed at the liquid–solid interface were studied. Calcofluor-binding polysaccharides (CBPS) and the complexes containing lipopolysaccharides (LPS) and proteinaceous structures were shown to fix mature biofilms on solid surfaces and to perform a framework function. The matrix of mature biofilms contained a carbohydrate hapten with a specific affinity to wheat germ agglutinin (WGA), which is important for associative interaction and plant root colonization. The carbohydrate components, predominating over the proteinaceous matrix components, mediate preferable biofilm attachment to hydrophilic surfaces, which is especially evident in the case of A. brasilense Sp245 mutants with impaired flagella formation. This is the first report on the presence of amyloid structures in the biomass of Azospirillum biofilms.

About the authors

A. V. Shelud’ko

Institute of Biochemistry and Physiology of Plants and Microorganisms, Russian Academy of Sciences

Author for correspondence.
Email: shel71@yandex.ru
Russian Federation, Saratov

Yu. A. Filip’echeva

Institute of Biochemistry and Physiology of Plants and Microorganisms, Russian Academy of Sciences

Email: shel71@yandex.ru
Russian Federation, Saratov

E. M. Telesheva

Institute of Biochemistry and Physiology of Plants and Microorganisms, Russian Academy of Sciences

Email: shel71@yandex.ru
Russian Federation, Saratov

A. M. Burov

Institute of Biochemistry and Physiology of Plants and Microorganisms, Russian Academy of Sciences

Email: shel71@yandex.ru
Russian Federation, Saratov

S. S. Evstigneeva

Institute of Biochemistry and Physiology of Plants and Microorganisms, Russian Academy of Sciences

Email: shel71@yandex.ru
Russian Federation, Saratov

G. L. Burygin

Institute of Biochemistry and Physiology of Plants and Microorganisms, Russian Academy of Sciences

Email: shel71@yandex.ru
Russian Federation, Saratov

L. P. Petrova

Institute of Biochemistry and Physiology of Plants and Microorganisms, Russian Academy of Sciences

Email: shel71@yandex.ru
Russian Federation, Saratov

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