Bacterial Diversity and Functional Activity of Microbial Communities in Hot Springs of the Baikal Rift Zone
- Authors: Lavrentyeva E.V.1,2, Radnagurueva A.A.1, Barkhutova D.D.1, Belkova N.L.3, Zaitseva S.V.1, Namsaraev Z.B.4, Gorlenko V.M.5, Namsaraev B.B.1
-
Affiliations:
- Institute of General and Experimental Biology, Siberian Branch
- Buryat State University
- Limnological Institute, Siberian Branch
- National Research Centre “Kurchatov Institute”
- The Federal Research Centre “Fundamentals of Biotechnology”
- Issue: Vol 87, No 2 (2018)
- Pages: 272-281
- Section: Experimental Articles
- URL: https://journals.rcsi.science/0026-2617/article/view/163463
- DOI: https://doi.org/10.1134/S0026261718020078
- ID: 163463
Cite item
Abstract
In this study the bacterial diversity of thermophilic microbial mats (40 to 65°C) in three alkaline hot springs of the Baikal Rift Zone (BRZ) was determined through pyrosequencing of 16S rRNA gene libraries. Significant diversity of bacterial species was found in the biomats of the hot springs with total number of detected phylotypes of 607. The highest share of the microbial community was represented by the phyla Chloroflexi (Seya Spring, 76.4%), Deinococcus-Thermus (Alla Spring, 45.1%), Nitrospira (Alla Spring, 36.1%), Cyanobacteria (Tsenkher Spring, 33.1%), and Proteobacteria (Tsenkher Spring, 22.6%), but their ratio varied significantly in different springs. A comparison of the biodiversity and composition of microbial communities between hot springs showed a decrease in biodiversity with increasing temperature. A large number of sequences showed a low degree of similarity with cultivated representatives in public databases. Microbial communities showed intensive rates of production and destruction of organic compounds, as revealed by the quantitative assessment of their functional activity.
About the authors
E. V. Lavrentyeva
Institute of General and Experimental Biology, Siberian Branch; Buryat State University
Author for correspondence.
Email: lena_l@mail.ru
Russian Federation, Ulan-Ude; Ulan-Ude
A. A. Radnagurueva
Institute of General and Experimental Biology, Siberian Branch
Email: lena_l@mail.ru
Russian Federation, Ulan-Ude
D. D. Barkhutova
Institute of General and Experimental Biology, Siberian Branch
Email: lena_l@mail.ru
Russian Federation, Ulan-Ude
N. L. Belkova
Limnological Institute, Siberian Branch
Email: lena_l@mail.ru
Russian Federation, Irkutsk
S. V. Zaitseva
Institute of General and Experimental Biology, Siberian Branch
Email: lena_l@mail.ru
Russian Federation, Ulan-Ude
Z. B. Namsaraev
National Research Centre “Kurchatov Institute”
Email: lena_l@mail.ru
Russian Federation, Moscow
V. M. Gorlenko
The Federal Research Centre “Fundamentals of Biotechnology”
Email: lena_l@mail.ru
Russian Federation, Moscow
B. B. Namsaraev
Institute of General and Experimental Biology, Siberian Branch
Email: lena_l@mail.ru
Russian Federation, Ulan-Ude
Supplementary files
