Inhibitory effect of aminoglycosides and tetracyclines on quorum sensing in Chromobacterium violaceum


如何引用文章

全文:

开放存取 开放存取
受限制的访问 ##reader.subscriptionAccessGranted##
受限制的访问 订阅存取

详细

Effect of aminoglycosides (kanamycin, gentamycin, and amikacin) and tetracyclines (tetracycline and doxycycline) on the CviI/CviR two-component quorum sensing mediated with C6-AHL autoinducer in Chromobacterium violaceum ATCC 31532 was studied. All antibiotics showed growth-inhibitory activity against the test strain and violated its density-dependent communication system in sub-inhibitory concentrations, which could be seen as suppression of C6-AHL-dependent violacein biosynthesis. The tested antibiotics exhibited an alternative balance of these activities: a direct antibacterial effect prevailed in tetracyclines, while aminoglycosides inhibited quorum sensing system of C. violaceum ATCC 31532 within a broad concentration range. Sensor strains capable of quantitative detection of C6-AHL and bearing the genes of resistance to aminoglycosides (C. violaceum 026) or tetracyclines (Escherichia coli pAL103) were used to analyze the effect of antibiotics on autoinducer accumulation in the cultivation medium. Aminoglycosides were found to suppress C6-AHL production within a broad concentration range; and therefore quorum sensing in C. violaceum ATCC 31532 did not function because of C6-AHL limitation. The obtained results were analyzed in relation to their biological role in natural microbiocenoses, as well as in relation to antibiotic treatment of bacterial infections caused by organisms using quorum sensing for induction of their pathogenic potential.

作者简介

D. Deryabin

All-Russian Research Institute of Beef Cattle Breeding

编辑信件的主要联系方式.
Email: dgderyabin@yandex.ru
俄罗斯联邦, Orenburg

K. Inchagova

All-Russian Research Institute of Beef Cattle Breeding

Email: dgderyabin@yandex.ru
俄罗斯联邦, Orenburg

补充文件

附件文件
动作
1. JATS XML

版权所有 © Pleiades Publishing, Ltd., 2018