Novel laccase—producing ascomycetes
- Авторлар: Myasoedova N.M.1, Renfeld Z.V.2, Podieiablonskaia E.V.1, Samoilova A.S.1, Chernykh A.M.1, Classen T.3, Pietruszka J.3,4, Kolomytseva M.P.1, Golovleva L.A.1
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Мекемелер:
- Skryabin Institute of Biochemistry and Physiology of Microorganisms
- Pushchino State University of Natural Sciences
- Institute of Bio- and Geosciences (IBG-1)
- Institute of Bioorganic Chemistry
- Шығарылым: Том 86, № 4 (2017)
- Беттер: 503-511
- Бөлім: Experimental Articles
- URL: https://journals.rcsi.science/0026-2617/article/view/163168
- DOI: https://doi.org/10.1134/S0026261717030110
- ID: 163168
Дәйексөз келтіру
Аннотация
Screening of ascomycetes producing laccases during growth on agar medium or submerged cultivation in the presence of various natural sources of carbon and energy (grain crops and potato) was carried out. The conditions of submerged cultivation of the most active strains (Myrothecium roridum VKM F-3565, Stachybotrys cylindrospora VKM F-3049, and Ulocladium atrum VKM F-4302) were optimized for the purpose of increasing laccase activity. The pH-optima and substrate selectivity of laccases in the culture liquid of the strains in relation to ABTS and phenolic compounds (2,6-dimethoxyphenol, syringaldazine, ferulic acid, p-coumaryl alcohol, and coniferyl alcohol) were investigated. High laccase activity at neutral pH was shown for the culture liquids of M. roridum VKM F-3565 and S. cylindrospora VKM F-3049 strains that provides prospects for using laccases of these strains in various cell biotechnologies.
Негізгі сөздер
Авторлар туралы
N. Myasoedova
Skryabin Institute of Biochemistry and Physiology of Microorganisms
Email: mkolomytseva@rambler.ru
Ресей, Pushchino
Zh. Renfeld
Pushchino State University of Natural Sciences
Email: mkolomytseva@rambler.ru
Ресей, Pushchino
E. Podieiablonskaia
Skryabin Institute of Biochemistry and Physiology of Microorganisms
Email: mkolomytseva@rambler.ru
Ресей, Pushchino
A. Samoilova
Skryabin Institute of Biochemistry and Physiology of Microorganisms
Email: mkolomytseva@rambler.ru
Ресей, Pushchino
A. Chernykh
Skryabin Institute of Biochemistry and Physiology of Microorganisms
Email: mkolomytseva@rambler.ru
Ресей, Pushchino
T. Classen
Institute of Bio- and Geosciences (IBG-1)
Email: mkolomytseva@rambler.ru
Германия, Berlin
J. Pietruszka
Institute of Bio- and Geosciences (IBG-1); Institute of Bioorganic Chemistry
Email: mkolomytseva@rambler.ru
Германия, Berlin; Düsseldorf
M. Kolomytseva
Skryabin Institute of Biochemistry and Physiology of Microorganisms
Хат алмасуға жауапты Автор.
Email: mkolomytseva@rambler.ru
Ресей, Pushchino
L. Golovleva
Skryabin Institute of Biochemistry and Physiology of Microorganisms
Email: mkolomytseva@rambler.ru
Ресей, Pushchino
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