Novel laccase—producing ascomycetes


Дәйексөз келтіру

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Аннотация

Screening of ascomycetes producing laccases during growth on agar medium or submerged cultivation in the presence of various natural sources of carbon and energy (grain crops and potato) was carried out. The conditions of submerged cultivation of the most active strains (Myrothecium roridum VKM F-3565, Stachybotrys cylindrospora VKM F-3049, and Ulocladium atrum VKM F-4302) were optimized for the purpose of increasing laccase activity. The pH-optima and substrate selectivity of laccases in the culture liquid of the strains in relation to ABTS and phenolic compounds (2,6-dimethoxyphenol, syringaldazine, ferulic acid, p-coumaryl alcohol, and coniferyl alcohol) were investigated. High laccase activity at neutral pH was shown for the culture liquids of M. roridum VKM F-3565 and S. cylindrospora VKM F-3049 strains that provides prospects for using laccases of these strains in various cell biotechnologies.

Авторлар туралы

N. Myasoedova

Skryabin Institute of Biochemistry and Physiology of Microorganisms

Email: mkolomytseva@rambler.ru
Ресей, Pushchino

Zh. Renfeld

Pushchino State University of Natural Sciences

Email: mkolomytseva@rambler.ru
Ресей, Pushchino

E. Podieiablonskaia

Skryabin Institute of Biochemistry and Physiology of Microorganisms

Email: mkolomytseva@rambler.ru
Ресей, Pushchino

A. Samoilova

Skryabin Institute of Biochemistry and Physiology of Microorganisms

Email: mkolomytseva@rambler.ru
Ресей, Pushchino

A. Chernykh

Skryabin Institute of Biochemistry and Physiology of Microorganisms

Email: mkolomytseva@rambler.ru
Ресей, Pushchino

T. Classen

Institute of Bio- and Geosciences (IBG-1)

Email: mkolomytseva@rambler.ru
Германия, Berlin

J. Pietruszka

Institute of Bio- and Geosciences (IBG-1); Institute of Bioorganic Chemistry

Email: mkolomytseva@rambler.ru
Германия, Berlin; Düsseldorf

M. Kolomytseva

Skryabin Institute of Biochemistry and Physiology of Microorganisms

Хат алмасуға жауапты Автор.
Email: mkolomytseva@rambler.ru
Ресей, Pushchino

L. Golovleva

Skryabin Institute of Biochemistry and Physiology of Microorganisms

Email: mkolomytseva@rambler.ru
Ресей, Pushchino

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© Pleiades Publishing, Ltd., 2017