Myrothecium verrucaria F-3851, a producer of laccases transforming phenolic compounds at neutral and alkaline conditions

Abstract

The conditions of submerged cultivation of the ascomycete Myrothecium verrucaria strain F-3851 were optimized in order to increase the yield of laccase in the culture liquid using the natural sources of carbon and energy (fresh rubbed potato tuber or floured grains of buckwheat, barley, oat, wheat, rye, rice, pea, or haricot). The pH-optima of oxidation of a number of laccase substrates (ABTS, 2,6-dimethoxyphenol, syringaldazine, ferulic acid, p-coumaryl alcohol, and coniferyl alcohol) by laccases of the culture liquid as well as substrate selectivity of laccases were investigated. The intermediates of transformation of phenylpropanoids (ferulic acid, p-coumaryl alcohol and coniferyl alcohol) by laccases of the culture liquid at neutral conditions were purified and identified. The ability of laccases of the culture liquid of M. verrucaria strain F-3851 to catalyze polymer compound formation during phenylpropanoid transformation was shown that offers the prospects of application of the laccases of M. verrucaria strain F-3851 for production of pharmacologically valuable polymers in a number of cellular biotechnologies carried out in neutral or alkaline environments.