Effect of O-acetylation of O antigen of Escherichia coli lipopolysaccharide on the nonspecific barrier function of the outer membrane
- Authors: Kulikov E.E.1, Majewska J.2, Prokhorov N.S.1, Golomidova A.K.1, Tatarskiy E.V.1, Letarov A.V.1
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Affiliations:
- Winogradsky Institute of Microbiology, Research Center of Biotechnology
- Hirszfeld Institute of Immunology and Experimental Therapy
- Issue: Vol 86, No 3 (2017)
- Pages: 310-316
- Section: Experimental Articles
- URL: https://journals.rcsi.science/0026-2617/article/view/163077
- DOI: https://doi.org/10.1134/S0026261717030080
- ID: 163077
Cite item
Abstract
Comparison of the methods for determination of permeability of the outer membrane of Escherichia coli strain 4s and its mutants was carried out. The studied isogenic strains E. coli 4s were obtained by selection of spontaneous mutants according to their sensitivity to bacteriophages recognizing the surface O antigen of the outer membrane lipopolysaccharide as a primary receptor. The variants differed in the presence and (de)acetylation of the lipopolysaccharide O antigen. A peptide antibiotic polymyxin, plasmid DNA, and lysozyme were used as probes. The role of acetylation of the O antigen of the lipopolysaccaride of E. coli outer membrane in modification of its permeability (correlating with bacteriophage sensitivity of the cells) was confirmed. Kinetic analysis using lysozyme was shown to be the optimal method for determination of the barrier function of E. coli outer membrane.
About the authors
E. E. Kulikov
Winogradsky Institute of Microbiology, Research Center of Biotechnology
Author for correspondence.
Email: eumenius@gmail.com
Russian Federation, Moscow
J. Majewska
Hirszfeld Institute of Immunology and Experimental Therapy
Email: eumenius@gmail.com
Poland, Polish
N. S. Prokhorov
Winogradsky Institute of Microbiology, Research Center of Biotechnology
Email: eumenius@gmail.com
Russian Federation, Moscow
A. K. Golomidova
Winogradsky Institute of Microbiology, Research Center of Biotechnology
Email: eumenius@gmail.com
Russian Federation, Moscow
E. V. Tatarskiy
Winogradsky Institute of Microbiology, Research Center of Biotechnology
Email: eumenius@gmail.com
Russian Federation, Moscow
A. V. Letarov
Winogradsky Institute of Microbiology, Research Center of Biotechnology
Email: eumenius@gmail.com
Russian Federation, Moscow
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