Effect of O-acetylation of O antigen of Escherichia coli lipopolysaccharide on the nonspecific barrier function of the outer membrane


如何引用文章

全文:

开放存取 开放存取
受限制的访问 ##reader.subscriptionAccessGranted##
受限制的访问 订阅存取

详细

Comparison of the methods for determination of permeability of the outer membrane of Escherichia coli strain 4s and its mutants was carried out. The studied isogenic strains E. coli 4s were obtained by selection of spontaneous mutants according to their sensitivity to bacteriophages recognizing the surface O antigen of the outer membrane lipopolysaccharide as a primary receptor. The variants differed in the presence and (de)acetylation of the lipopolysaccharide O antigen. A peptide antibiotic polymyxin, plasmid DNA, and lysozyme were used as probes. The role of acetylation of the O antigen of the lipopolysaccaride of E. coli outer membrane in modification of its permeability (correlating with bacteriophage sensitivity of the cells) was confirmed. Kinetic analysis using lysozyme was shown to be the optimal method for determination of the barrier function of E. coli outer membrane.

作者简介

E. Kulikov

Winogradsky Institute of Microbiology, Research Center of Biotechnology

编辑信件的主要联系方式.
Email: eumenius@gmail.com
俄罗斯联邦, Moscow

J. Majewska

Hirszfeld Institute of Immunology and Experimental Therapy

Email: eumenius@gmail.com
波兰, Polish

N. Prokhorov

Winogradsky Institute of Microbiology, Research Center of Biotechnology

Email: eumenius@gmail.com
俄罗斯联邦, Moscow

A. Golomidova

Winogradsky Institute of Microbiology, Research Center of Biotechnology

Email: eumenius@gmail.com
俄罗斯联邦, Moscow

E. Tatarskiy

Winogradsky Institute of Microbiology, Research Center of Biotechnology

Email: eumenius@gmail.com
俄罗斯联邦, Moscow

A. Letarov

Winogradsky Institute of Microbiology, Research Center of Biotechnology

Email: eumenius@gmail.com
俄罗斯联邦, Moscow

补充文件

附件文件
动作
1. JATS XML

版权所有 © Pleiades Publishing, Ltd., 2017