Broiler chicken cecal microbiocenoses depending on mixed fodder

V. I. Fisinin; L. A. Il’ina; E. A. Iyldyrym; I. N. Nikonov; V. A. Filippova; G. Yu. Laptev; N. I. Novikova; A. A. Grozina; T. N. Lenkova; V. A. Manukyan; I. A. Egorov

doi:10.1134/S0026261716040056

Broiler chicken cecal microbiocenoses depending on mixed fodder

Authors: Fisinin V.I.¹, Il’ina L.A.², Iyldyrym E.A.², Nikonov I.N.², Filippova V.A.², Laptev G.Y.², Novikova N.I.², Grozina A.A.¹, Lenkova T.N.¹, Manukyan V.A.¹, Egorov I.A.¹
Affiliations:
1. All-Russian Research and Technological Institute of Aviculture
2. BIOTROF+ Ltd
Issue: Vol 85, No 4 (2016)
Pages: 493-499
Section: Experimental Articles
URL: https://journals.rcsi.science/0026-2617/article/view/162745
DOI: https://doi.org/10.1134/S0026261716040056
ID: 162745

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Abstract

Molecular genetic techniques (NGS sequencing and quantitative PCR) were used to determine the composition of the cecal bacterial community of broiler chickens fed with different mixed fodder. The cecal microbiome exhibited taxonomic diversity, with both typical inhabitants of avian intestine belonging to the families Clostridiaceae, Eubacteriaceae, and Lactobacillaceae and to the phylum Bacteroidetes, and new unidentified taxa, as well as bacteria of the families Lachnospiraceae and Ruminococcaceae, which were previously considered restricted to the rumen microbiota. Contrary to traditional concepts, enterococci and bifidobacteria were among the minor components of the community, lactate-fermenting species were absent, and typical avian pathogens of the genus Staphylococcus were detected but seldom. Members of the family Suterellaceae and the genus Gallibacterium, which are responsible for avian respiratory infections, were also detected. Significant fluctuations of abundance and composition of microbial groups within the cecal community and of the parameters of broiler productivity were found to occur depending on the feed allowance. Cellulose content in the feed had the most pronounced effect on the composition and structure of bacterial communities. Decreased cellulose content resulted in a decrease of bacterial abundance by an order of magnitude and in increased ratios of members of the phylum Bacteroidetes and the family Clostridiaceae, which possess the enzymes degrading starch polysaccharides. Abundance of the normal inhabitants of avian intestine belonging to the genus Lactobacillus and the order Bacillales decreased, while the share of Escherichia and members of the family Sutterellaceae increased, including some species capable of causing dysbiotic changes in the avian intestine. No significant change in the abundance of cellulolytics of the families Ruminococcaceae, Lachnospiraceae, and Eubacteriaceae was observed.

Keywords

intestinal microbiota, ceces, broiler chickens, molecular genetic techniques, NGS sequencing, quantitative PCR

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Broiler chicken cecal microbiocenoses depending on mixed fodder

Full Text

Abstract

Keywords

About the authors

Supplementary files