Copper ions’ influence on thiocyonate dehydrogenase packing and conformation in a crystal
- Authors: Varfolomeeva L.А.1, Solovieva A.Y.1, Shipkov N.S.1, Dergousova N.I.1, Minyaev М.E.2, Boyko K.M.1, Tikhonova T.V.1, Popov V.O.1
-
Affiliations:
- Federal Research Centre “Fundamentals of Biotechnology” of the Russian Academy of Sciences
- N.D. Zelinsky Institute of Organic Chemistry of the Russian Academy of Sciences
- Issue: Vol 70, No 1 (2025)
- Pages: 10-17
- Section: STRUCTURE OF MACROMOLECULAR COMPOUNDS
- URL: https://journals.rcsi.science/0023-4761/article/view/286223
- DOI: https://doi.org/10.31857/S0023476125010027
- EDN: https://elibrary.ru/IUBQXT
- ID: 286223
Cite item
Abstract
The copper-containing enzyme thiocyanate dehydrogenase (TcDH) catalyzes oxidation of thiocyanate to cyanate and elemental sulfur. To date, the spatial structures of two bacterial TcDHs (tpTcDH and pmTcDH) are known. Both enzymes are dimers and contain a trinuclear copper center in the active site. The important difference between these enzymes is that in a crystal, the subunits of the tpTcDH dimer are in identical conformations, while the subunits of the pmTcDH dimer are in different conformations: closed and open. To clarify the role of copper ions in changing the TcDH conformation, the structure of the apo-form of pmTcDH was established, in which both subunits of the dimer had the closed conformation. Soaking of apo-form crystals with copper led to the restoring of the trinuclear center and the conformational rearrangements of the subunits.
Full Text

About the authors
L. А. Varfolomeeva
Federal Research Centre “Fundamentals of Biotechnology” of the Russian Academy of Sciences
Author for correspondence.
Email: l.varfolomeeva@fbras.ru
Russian Federation, Moscow
A. Y. Solovieva
Federal Research Centre “Fundamentals of Biotechnology” of the Russian Academy of Sciences
Email: l.varfolomeeva@fbras.ru
Russian Federation, Moscow
N. S. Shipkov
Federal Research Centre “Fundamentals of Biotechnology” of the Russian Academy of Sciences
Email: l.varfolomeeva@fbras.ru
Russian Federation, Moscow
N. I. Dergousova
Federal Research Centre “Fundamentals of Biotechnology” of the Russian Academy of Sciences
Email: l.varfolomeeva@fbras.ru
Russian Federation, Moscow
М. E. Minyaev
N.D. Zelinsky Institute of Organic Chemistry of the Russian Academy of Sciences
Email: l.varfolomeeva@fbras.ru
Russian Federation, Moscow
K. M. Boyko
Federal Research Centre “Fundamentals of Biotechnology” of the Russian Academy of Sciences
Email: l.varfolomeeva@fbras.ru
Russian Federation, Moscow
T. V. Tikhonova
Federal Research Centre “Fundamentals of Biotechnology” of the Russian Academy of Sciences
Email: l.varfolomeeva@fbras.ru
Russian Federation, Moscow
V. O. Popov
Federal Research Centre “Fundamentals of Biotechnology” of the Russian Academy of Sciences
Email: l.varfolomeeva@fbras.ru
Russian Federation, Moscow
References
- Sorokin D.Y., Tourova T.P., Lysenko A.M. et al. // Int. J. Syst. Evol. Microbiol. 2002. V. 52. Pt 2. P. 657. http://dx.doi.org/10.1099/00207713-52-2-657
- Slobodkina G.B., Merkel A.Y., Novikov A.A. et al. // Extremophiles. 2020. V. 24. № 1. P. 177. http://dx.doi.org/10.1007/s00792-019-01145-0
- Tikhonova T.V., Sorokin D.Y., Hagen W.R. et al. // Proc. Natl. Acad. Sci. USA. 2020. V. 117. № 10. P. 5280. http://dx.doi.org/10.1073/pnas.1922133117
- Varfolomeeva L.A., Shipkov N.S., Dergousova N.I. et al. // Int. J. Biol. Macromol. 2024. P. 135058. http://dx.doi.org/10.1016/j.ijbiomac.2024.135058
- Varfolomeeva L.A., Solovieva A.Y., Shipkov N.S. et al. // Crystals. 2022. V. 12. P. 1787. http://dx.doi.org/10.3390/cryst12121787
- Varfolomeeva L.A., Polyakov K.M., Komolov A.S. et al. // Crystallography Reports. 2023. V. 68. № 6. P. 886. http://dx.doi.org/10.1134/s1063774523600990
- McPherson A. // Methods Mol. Biol. 2017. V. 1607. P. 17. http://dx.doi.org/10.1007/978-1-4939-7000-1_2
- Atakisi H., Moreau D.W., Thorne R.E. // Acta Cryst. D. 2018. V. 74. № 4. P. 264. http://dx.doi.org/10.1107/S2059798318000207
- Kishan K.V., Zeelen J.P., Noble M.E. et al. // Protein Sci. 1994. V. 3. № 5. P. 779. http://dx.doi.org/10.1002/pro.5560030507
- Kovari Z., Vas M. // Proteins. 2004. V. 55. № 1. P. 198. http://dx.doi.org/10.1002/prot.10469
- Hakansson K., Doherty A.J., Shuman S., Wigley D.B. // Cell. 1997. V. 89. № 4. P. 545. http://dx.doi.org/10.1016/s0092-8674(00)80236-6
- Lamzin V.S., Dauter Z., Popov V.O. et al. // J. Mol. Biol. 1994. V. 236. № 3. P. 759. http://dx.doi.org/10.1006/jmbi.1994.1188
- Kabsch W. // Acta Cryst. D. 2010. V. 66. № 2. P. 125. http://dx.doi.org/10.1107/S0907444909047337
- Agirre J., Atanasova M., Bagdonas H. et al. // Acta Cryst. D. 2023. V. 79. № 6. P. 449. http://dx.doi.org/10.1107/S2059798323003595
- Vagin A., Teplyakov A. // Acta Cryst. D. 2010. V. 66. № 1. P. 22. http://dx.doi.org/10.1107/S0907444909042589
- Murshudov G.N., Skubak P., Lebedev A.A. et al. // Acta Cryst. D. 2011. V. 67. № 4. P. 355. http://dx.doi.org/10.1107/S0907444911001314
- Emsley P., Lohkamp B., Scott W.G., Cowtan K. // Acta Cryst. D. 2010. V. 66. № 4. P. 486. http://dx.doi.org/10.1107/S0907444910007493
- Krissinel E., Henrick K. // J. Mol. Biol. 2007. V. 372. № 3. P. 774. http://dx.doi.org/10.1016/j.jmb.2007.05.022
- Kabsch W. // Acta Cryst. A. 1976. V. 32. № 5. P. 922. http://dx.doi.org/10.1107/S0567739476001873
- Appel M.J., Meier K.K., Lafrance-Vanasse J. et al. // Proc. Natl. Acad. Sci. U S A. 2019. V. 116. № 12. P. 5370. http://dx.doi.org/10.1073/pnas.1818274116
- Osipov E.M., Polyakov K.M., Tikhonova T.V. et al. // Acta Cryst. F. 2015. V. 71. № 12. P. 1465. http://dx.doi.org/10.1107/S2053230X1502052X
Supplementary files
