Preliminary X-ray Diffraction Analysis of Purine Nucleoside Phosphorylase from the Haloalkaliphilic Bacterium Halomonas chromatireducens

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Crystals of the enzyme purine nucleoside phosphorylase from the extremophilic bacterium Halomonas Chromatireducens AGD 8-3, suitable for X-ray diffraction, were grown by the vapor-diffusion method. The X-ray diffraction data were collected from these crystals at the Belok beamline of the Kurchatov synchrotron radiation source (National Research Centre “Kurchatov Institute”) at 100 K to 1.80 Å resolution. The X-ray diffraction data were processed in the space groups P1, P2, P21, and P622. The structure was solved by the molecular replacement method taking into account the twinning in the space groups P21 and P1 with one and two hexamers of the enzyme per asymmetric unit, respectively.

作者简介

T. Safonova

Bach Institute of Biochemistry, Federal Research Centre “Fundamentals of Biotechnology,” Russian Academy of Sciences, 119071, Moscow, Russia

Email: tn_safonova@mail.ru
Россия, Москва

A. Antipov

Bach Institute of Biochemistry, Federal Research Centre “Fundamentals of Biotechnology,” Russian Academy of Sciences, 119071, Moscow, Russia

Email: kmpolyakov@gmail.com
Россия, Москва

V. Veiko

Bach Institute of Biochemistry, Federal Research Centre “Fundamentals of Biotechnology,” Russian Academy of Sciences, 119071, Moscow, Russia

Email: kmpolyakov@gmail.com
Россия, Москва

N. Mordkovich

Bach Institute of Biochemistry, Federal Research Centre “Fundamentals of Biotechnology,” Russian Academy of Sciences, 119071, Moscow, Russia; Bochkov Research Centre for Medical Genetics, 115522, Moscow, Russia

Email: kmpolyakov@gmail.com
Россия, Москва; Россия, Москва

N Okorokova

Bach Institute of Biochemistry, Federal Research Centre “Fundamentals of Biotechnology,” Russian Academy of Sciences, 119071, Moscow, Russia

Email: kmpolyakov@gmail.com
Россия, Москва

P. Dorovatovskii

National Research Centre “Kurchatov Institute”, 123098, Moscow, Russia

Email: kmpolyakov@gmail.com
Россия, Москва

K. Polyakov

Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, 119991, Moscow, Russia

编辑信件的主要联系方式.
Email: kmpolyakov@gmail.com
Россия, Москва

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版权所有 © Т.Н. Сафонова, А.Н. Антипов, В.П. Вейко, Н.Н. Мордкович, Н.А. Окорокова, П.В. Дороватовский, К.М. Поляков, 2023

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